Study of the hypolipidemic properties of pectin, garlic and ginseng in hypercholesterolemic rabbits
Study of the hypolipidemic properties of pectin, garlic and ginseng in hypercholesterolemic rabbits
复制标题
DOI：
10.1006/phrs.1998.0421
复制
发表时间：
1999-02-01
影响因子：
9.3
通讯作者：
Hamdy, MA
中科院分区：
文献类型：
--
作者：
Ismail, MF;Gad, MZ;Hamdy, MA
研究了实验性高胆固醇血症及其通过一些天然膳食补充剂（果胶、大蒜和人参）和药物吉非罗齐的调节作用。实验性高胆固醇血症是通过给兔子喂食2%胆固醇富集的饮食28天来诱导的。高胆固醇血症家兔分为5组。一组不接受治疗，作为高胆固醇血症对照组。其他四组喂食富含胆固醇的饮食，同时每日口服10%果胶、2%大蒜、2%人参或135 mg g（-1）B.w吉非罗齐。研究中还包括饲喂普通饲料的正常家兔组。通过估计血清甘油三酯（TG）、总胆固醇、LDL和HDL胆固醇来检查上述治疗的降血脂作用。在静脉注射肝素（200 IU/kg（-1）B.w）后10分钟获得的肝素后血浆中估计肝素后总活性和肝脂肪酶活性。测定血浆丙二醛（MDA）含量和红细胞超氧化物歧化酶（SOD）活性，评价家兔的抗氧化状况。处死后取主动脉行组织病理学检查。研究结果表明，喂食富含胆固醇的饮食导致总胆固醇、LDL胆固醇和HDL胆固醇、血浆MDA以及肝素后总脂酶和肝脂酶活性显著增加。而血清TG和红细胞SOD则无明显变化。组织学检查发现主动脉壁有明显改变，出现大量粥样斑块。大蒜和果胶都成功地显著降低了高胆固醇血症，与吉非罗齐相当。大蒜是唯一具有抗脂质过氧化特性的治疗。红细胞SOD活性不受高胆固醇血症或任何治疗。此外，没有一种治疗能够改变与高胆固醇血症相关的肝素后脂解活性的显著升高或显著影响血清甘油三酯水平。最后，在接受治疗的高胆固醇血症组中，吉非罗齐组动物的主动脉壁变化最小。在用果胶或大蒜处理的动物的主动脉中观察到轻微变性。人参给药未能对与富含胆固醇的饮食相关的显著的高胆固醇血症或动脉粥样硬化发挥任何显著的保护作用。(C)1999年意大利药理学会。
Experimental hypercholesterolemia and its modulation by some natural dietary supplements (pectin, garlic and ginseng) and by the drug gemfibrozil were studied. Experimental hypercholesterolemia was induced by feeding rabbits a 2% cholesterol-enriched diet for 28 days. Hypercholesterolemic rabbits were classified into five groups. One group did not receive treatments and served as a control hypercholesterolemic group. The other four groups were fed the cholesterol-enriched diet in conjunction with either 10% pectin, 2% garlic, 2% ginseng or 135 mg g(-1) b.w gemfibrozil in a daily oral dose. A normal group of rabbits fed a plain chow diet was also included in the study. The hypolipidemic effect of the above treatments was examined by estimating serum triglycerides (TG), total-, LDL- and HDL-cholesterol. Post-heparin total and hepatic lipase activities were estimated in postheparin plasma obtained 10 min after an intravenous injection of heparin (200 IU kg(-1) b.w). In order to evaluate the antioxidant status of the rabbits, plasma malondialdehyde (MDA) level and erythrocyte superoxide dismutase (SOD) activity were measured. After killing, aorta from all rabbits were subjected to histopathological examination. Results of the study demonstrated that feeding the cholesterol-enriched diet caused a significant increase in total-, LDL-, and HDL-cholesterol, plasma MDA and post-heparin total and hepatic lipase activities. On the other hand, serum TG and erythrocyte SOD were not changed. Histopathological examination revealed marked alteration in the aortic wall with the appearance of large multiple atheromatous plaques. Both garlic and pectin were successful in a significant reduction of the hypercholesterolemia in a way comparable to gemfibrozil. Garlic was the only treatment that has antilipid peroxidative property. Erythrocyte SOD activity was not affected by hypercholesterolemia or by any of the treatments. Also, none of the treatments were able to modify the significant elevation of post-heparin lipolytic activities associated with the hypercholesterolemia or to significantly affect the serum triglycerides level. Finally, among the hypercholesterolemic groups that received treatments, the least changes in the aortic wall were shown in the animals of the gemfibrozil group. Slight degeneration was observed in the aorta of animals treated with pectin or garlic. Ginseng administration failed to exert any significant protection from the remarkable hypercholesterolemia or atherosclerosis associated with the cholesterol-enriched diet. (C) 1999 The Italian Pharmacological Society.