Specific proteins associated with Creutzfeldt-Jakob disease and scrapie share antigenic and carbohydrate determinants.
Specific proteins associated with Creutzfeldt-Jakob disease and scrapie share antigenic and carbohydrate determinants.
复制标题
与克雅氏病和痒病相关的特定蛋白质具有共同的抗原和碳水化合物决定簇。
DOI：
10.1073/pnas.82.12.4263
复制
发表时间：
1985
影响因子：
11.1
通讯作者：
Manuelidis,EE
中科院分区：
文献类型：
--
作者：
Manuelidis,L;Valley,S;Manuelidis,EE
感染克雅氏病(CJD)的少量脑组织(2克)可以用简单的1天法分离，其中包括N-月桂酰肌氨酸裂解。以前已经在瘙痒病和CJD感染的组织中发现了独特的纤维。这些纤维在最终组分中含量丰富。从人类CJD尸检材料、实验仓鼠和豚鼠CJD制备的样品都显示出在对照样品中看不到的易于识别的纤维。因此，这些方法在疑似人类CJD病例的活检诊断中是有价值的。用N-月桂酰肌氨酸裂解可定量溶解富膜组份的感染性。在微量分离中恢复了显著的传染性。经K酶消化后，在NaDodSO4/PAGE上可检测到一条29 kDa的弥散条带。这种29 kDa的物质在未感染的对照组大脑中不存在，与在瘙痒病中看到的类似。人、豚鼠和仓鼠CJD组分的蛋白印迹用一种针对小鼠瘙痒病29 kDa条带的抗体进行检测；29 kDa的蛋白在所有CJD和瘙痒病组分中都有标记，但在对照组中没有标记。这些结果表明，这两种疾病中的特定蛋白具有共同的抗原决定簇。蓖麻毒素和小麦胚芽凝集素，但不是刀豆蛋白A，也标记了来自仓鼠CJD和仓鼠瘙痒组分的29 kDa条带的一部分，但他们没有标记相同凝胶蛋白负荷下正常仓鼠组分的任何条带。当省略蛋白酶K处理时，在CJD样品中检测到约等于35 kDa的特异性条带。这些结果与某些CJD和瘙痒病特异蛋白是糖蛋白或唾液酸糖蛋白的观点一致，这些蛋白可以驻留在细胞膜中或可能来自细胞膜。
Small amounts of brain tissue (2 g) infected with Creutzfeldt-Jakob disease (CJD) can be fractionated by using a simple 1-day method that includes lysis with N-lauroylsarcosine. Unique fibrils have been identified previously in scrapie- and CJD-infected tissue. These fibrils were abundant in final fractions. Preparations from human CJD autopsy material and from experimental hamster and guinea pig CJD all displayed readily identifiable fibrils that were not seen in control preparations. Thus, these methods appear to be of value in biopsy diagnosis of suspected human cases of CJD. Lysis with N-lauroylsarcosine quantitatively solubilized infectivity from membrane-rich fractions. Significant infectivity was recovered in microfractionations. After proteinase K digestion, a diffuse band at 29 kDa was detectable on NaDodSO4/PAGE. This 29-kDa material was not present in uninfected control brain and was similar to that seen in scrapie. Protein blots of human, guinea pig, and hamster CJD fractions were tested with an antibody raised against a 29-kDa band from mouse scrapie; 29-kDa proteins were labeled in all CJD and scrapie fractions but not in controls. These results indicate that specific proteins in both these diseases share common antigenic determinants. Ricin and wheat germ agglutinin, but not concanavalin A, also labeled a portion of the 29-kDa band from hamster CJD and hamster scrapie fractions, but they did not label any bands in normal hamster fractions at the same gel protein loads. When proteinase K treatment was omitted, specific bands of approximately equal to 35 kDa were detected in CJD samples. These results are consistent with the idea that some CJD- and scrapie-specific proteins are glycoproteins or sialoglycoproteins that can reside in or possibly derive from cell membranes.
登录
查看更多内容
中枢神经系统的慢病毒感染，ter Meulen V. Würzburg, Katz M. (Eds.)，见：这些疾病的病因学和发病机制的研究方法，牛津 (1977)，Cloth DM 62,50， 27.50 美元
影响因子：
3.9
作者：
J. Panouse
通讯作者：
J. Panouse
阿尔茨海默病的生物学方面
DOI：
--
发表时间：
1983
期刊：
影响因子：
--
作者：
R. Katzman
通讯作者：
R. Katzman
Banbury Report 15，阿尔茨海默病的生物学方面：R. Katzman 编辑，冷泉港实验室，1983 年。55.00 美元（美国境外 66.00 美元）（xiv 495 页）ISBN 0 87969 213 8
DOI：
--
发表时间：
1984
期刊：
影响因子：
--
作者：
G. Reynolds
通讯作者：
G. Reynolds
神经递质和药物，第二版
DOI：
--
发表时间：
1984
期刊：
影响因子：
--
作者：
Eamonn Kelly
通讯作者：
Eamonn Kelly