Retrospective diagnostics of congenital cytomegalovirus infection performed by polymerase chain reaction in blood stored on filter paper
Retrospective diagnostics of congenital cytomegalovirus infection performed by polymerase chain reaction in blood stored on filter paper
复制标题
DOI：
10.3109/00365549709011855
复制
发表时间：
1997-01-01
影响因子：
--
通讯作者：
Guthenberg, C
中科院分区：
文献类型：
--
作者：
Johansson, PJH;Jonsson, M;Guthenberg, C
对63例出生后3-7天的新生儿毛细血管血标本，用聚合酶链反应（PCR）法检测CMV DNA，并将其滤纸片保存12-18年。对16例确诊为先天性CMV感染的新生儿进行了CMV DNA检测13例（81%）CMV PCR检测阳性，3例（19%）PCR检测阴性。来自16名没有先天性CMV感染的对照婴儿的所有血液样本（在1周龄内采样的尿液中的病毒分离测试为阴性）均为CMV PCR阴性。当31份滤纸样本被放置在感染婴儿样本的上方或下方时，6份（19%）具有弱反应性。这表明CMV DNA可以在储存期间从一张滤纸转移到另一张滤纸上。我们的结论是，在滤纸上储存的干血上进行PCR是一种有用的方法，在先天性CMV感染的回顾性诊断，但必须考虑到，从附近储存的血液样本中转移CMV DNA的可能性。
Capillary blood samples from 63 infants collected 3-7 days after birth, and thereafter stored on filter papers for 12-18 y, mere tested for the presence of CMV DNA by the polymerase chain reaction (PCR) method, Of 16 infants with proven congenital CMV infection (positive virus isolation test in urine sampled within 1 meek of age), 13 (81%) had a positive CMV PCR test and 3 (19%) a negative PCR test. All blood samples from 16 control infants without congenital CMV infection (negative virus isolation test in urine sampled within 1 week of age) were CMV PCR-negative. When 31 samples on filter papers stored above or below the samples of the infected infants were tested, 6 (19%) had a weak reactivity. This suggests that CMV DNA can be transferred from one filter paper to another during storage. We conclude that PCR performed on dried blood stored on filter paper is a useful method in the retrospective diagnostics of congenital CMV infection, Consideration must be given, however, to the possibility of transfer of CMV DNA from blood samples stored nearby.