A functional involvement of ABCE1, eukaryotic ribosome recycling factor, in nonstop mRNA decay in Drosophila melanogaster cells
A functional involvement of ABCE1, eukaryotic ribosome recycling factor, in nonstop mRNA decay in Drosophila melanogaster cells
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DOI：
10.1016/j.biochi.2014.08.001
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发表时间：
2014-11-01
期刊：
影响因子：
3.9
通讯作者：
Inada, Toshifumu
中科院分区：
文献类型：
--
作者：
Kashima, Isao;Takahashi, Masaki;Inada, Toshifumu
当核糖体遇到缺乏停止密码子的mRNA时，两种质量控制机制，即用于不间断mRNA衰变的NSD和用于蛋白酶体对不间断蛋白的共翻译降解的核糖体质量控制（RQC），被触发以消除异常分子。在酵母中，已知Dom34 （eRF1的同源物）和Ltn1 （E3泛素连接酶）分别通过在不间断mrna的3'端触发核糖体拯救和蛋白酶体依赖性多肽降解，在NSD和RQC中发挥关键作用。本研究证实了Ltn1在果蝇细胞不间断产物RQC中的重要作用，并进一步揭示了ABCE1（一种真核核糖体回收因子）在果蝇细胞NSD中的功能作用。(C) 2014 Elsevier B.V.和法国生物化学与生物分子学会（SFBBM）。版权所有。
When ribosomes encounter mRNAs lacking stop codons, two quality-control machineries, NSD for nonstop mRNA decay and ribosome quality control (RQC) for co-translational degradation of the nonstop protein by the proteasome, are triggered to eliminate aberrant molecules. In yeast, it is known that Dom34 (a homolog of eRF1) and Ltn1 (an E3 ubiquitin ligase) play crucial roles in NSD and RQC, respectively, by triggering ribosome rescue at the 3' end of nonstop mRNAs and proteasome-dependent polypeptide degradation. Here we confirmed the essential role of Ltn1 in RQC for nonstop products in Drosophila cells, and further uncovered a functional role of ABCE1, a eukaryotic ribosome recycling factor, in NSD in Drosophila cells. (C) 2014 Elsevier B.V. and Societe francaise de biochimie et biologie Moleculaire (SFBBM). All rights reserved.