Systemic tolerance and secretory immunity after oral immunization.
Systemic tolerance and secretory immunity after oral immunization.
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DOI：
10.1084/jem.152.6.1459
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发表时间：
1980-12-01
期刊：
影响因子：
--
通讯作者：
Tomasi TB Jr
中科院分区：
文献类型：
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作者：
Challacombe SJ;Tomasi TB Jr
在几种动物模型中已经报道了摄入抗原后全身免疫反应减弱。相反，最近有报道称，口服免疫可能导致分泌性抗体的产生。为了确定这些事件是否可以同时发生，CBA/J小鼠用不同剂量的卵清蛋白（OVA）和变形链球菌灌胃免疫。7天后，用完全佐剂中的抗原对动物进行全身攻击，8天后采集血清和唾液，并测定引流淋巴结的增殖反应。胃内剂量为1 mg OVA或10（9）S。10 mg OVA或2.5 × 10（9）S灌胃给药可显著抑制增殖反应。变形杆菌导致使用血凝反应产生可检测的唾液抗体。灌胃OVA或S.变异体OVA灌胃后2-60 d，S.变异人用异源抗原预先胃内免疫不能抑制对OVA或S.变异体从给予20 mg OVA或10（9）S的小鼠中转移40 × 10（6）个肠系膜淋巴结细胞。变形杆菌导致同基因受体中增殖反应的抑制。唾液中的抗体被抗IgA抗体吸收而被清除，但抗IgG或IgM抗体不能被清除，这表明它们属于伊加类。看来，小鼠灌胃给予可溶性或颗粒性抗原可能导致同时诱导唾液抗体和全身抑制。
Diminished systemic immune reaction after ingestion of antigen has been reported in several animal models. Conversely, it has been reported recently that oral immunization may lead to the production of secretory antibodies. To determine whether these events could occur concurrently, CBA/J mice were immunized intragastrically with varying doses of ovalbumin (OVA) and Streptococcus mutans. After 7 d, the animals were challenged systemically with antigen in complete adjuvant and 8 d later serum and saliva taken, and the draining lymph nodes assayed for a proliferative response. Intragastric doses of 1 mg OVA or 10(9) S. mutans led to significant suppression of the proliferative response, and intragastric doses of 10 mg OVA or 2.5 X 10(9) S. mutans led to the production of detectable salivary antibodies using hemagglutination. Serum antibodies were not detected after intragastric administration of OVA or S. mutans. Suppression of the proliferative response could be detected from 2-60 d after intragastric administration of OVA, and 2-21 d after S. mutans. Prior intragastric immunization with heterologous antigens did not suppress the response to OVA or S. mutans. Transfer of 40 X 10(6) mesenteric lymph node cells from mice given 20 mg OVA or 10(9) S. mutans led to suppression of the proliferative response in syngeneic recipients. Salivary antibodies wer removed by absorption with anti-IgA, but not anti-IgG or IgM, indicating that they were of the IgA class. It appears that intragastric administration of soluble or particulate antigens in mice may lead to the concurrent induction of salivary antibodies and systemic suppression.