IL-15 enhances the function and inhibits CD95/Fas-induced apoptosis of human CD4+ and CD8+ effector-memory T cells
IL-15 enhances the function and inhibits CD95/Fas-induced apoptosis of human CD4+ and CD8+ effector-memory T cells
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DOI：
10.1093/intimm/dxg013
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发表时间：
2003-01-01
影响因子：
4.4
通讯作者：
Katsikis, PD
中科院分区：
文献类型：
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作者：
Mueller, YM;Makar, V;Katsikis, PD
尽管IL-15在体外和体内对小鼠细胞的影响已经被描述，但它对人类记忆CD8(+)T细胞的影响还没有很好的表征。我们在这里表明，IL-15优先增强健康和HIV感染者的人类效应记忆CD45RA(-)、CD62L(-)和CD45RA(+)、CD62L(-)、CD4(+)和CD8(+)T细胞的激活和效应功能。我们发现，IL-15使抗CD3刺激的纯化的CD4(+)和CD8(+)T细胞以及来自健康和HIV感染者的外周血单核细胞对效应细胞因子干扰素-γ和肿瘤坏死因子-α的激活和分泌增加了2-5倍。此外，IL-15有效地抑制了CD95/Fas诱导的HIV感染者效应记忆CD4(+)和CD8(+)T细胞的凋亡。这些发现表明，IL-15除了是记忆性CD8(+)T细胞的生长和存活因子外，在健康人和HIV感染者中也是人类效应-记忆性CD8(+)T细胞的有效激活剂。
Although the effect of IL-15 has been described on murine cells in vitro and in vivo, its effect on human memory CD8(+) T cells is not well characterized. We show here that IL-15 preferentially enhances the activation and effector function of human effector-memory CD45RA(-)CD62L(-) and CD45RA(+)CD62L(-)CD4(+) and CD8(+) T cells in both healthy and HIV-infected individuals. We find that IL-15 increases 2- to 5-fold both the activation and secretion of the effector cytokines IFN-gamma and tumor necrosis factor (TNF)-alpha by anti-CD3-stimulated purified CD4(+) and CD8(+) T cells and peripheral blood mononuclear cells from healthy and HIV-infected individuals. Furthermore, IL-15 potently inhibits CD95/Fas-induced apoptosis of the effector-memory CD4(+) and CD8(+) T cells from HIV-infected individuals. These findings suggest that in addition to being a growth and survival factor for memory CD8(+) T cells, IL-15 is also a potent activator of human effector-memory CD8(+) T cells both in healthy and in HIV-infected individuals.