Immunologic Mechanism of Patchouli Alcohol Anti-H1N1 Influenza Virus May Through Regulation of the RLH Signal Pathway In Vitro
Immunologic Mechanism of Patchouli Alcohol Anti-H1N1 Influenza Virus May Through Regulation of the RLH Signal Pathway In Vitro
复制标题
广藿香醇抗H1N1流感病毒的体外免疫机制可能通过调节RLH信号通路
DOI：
10.1007/s00284-013-0381-y
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发表时间：
2013-10-01
影响因子：
2.6
通讯作者：
Chen, Xiao-Yin
中科院分区：
文献类型：
--
作者：
Wu, Xian-Lin;Ju, Da-Hong;Chen, Xiao-Yin
广藿香醇是从中药广藿香中提取的一种醇类化合物。本研究旨在观察PA体外抗流感病毒的作用。用甲型H1N1流感病毒（A/FM 1/1/47）感染16 HBE（人呼吸道上皮细胞）建立细胞模型。然后将16 HBE与树突状细胞、巨噬细胞和单核细胞共培养，加入PA（浓度为10 μg/mL）作为处理干预24 h。收集免疫细胞和上清液进行RT-PCR和ELISA检测RLH（RIG-1-likehelicases）通路相关蛋白。结果表明，H1N1感染后，细胞培养上清中IL-4和IFN-γ水平升高，PA处理抑制细胞因子和RLH通路mRNA的表达。PA抗流感病毒可能通过调节RLH信号通路。
AbstractPatchouli alcohol (PA) is a kind of methanol extracted from traditional Chinese medicine Pogostemonis Herba. Our research aimed to observe the anti-influenza virus role of PA in vitro. 16HBE (human respiratory epithelial cell) was infected by H1N1 (A/FM1/1/47) to set the cell model. Then the 16HBE was co-cultivated with three kinds of immune cells: dendritic cells, macrophages, and monocytes, PA (the concentration is 10 μg/mL) was added as a treatment intervention for 24 h. The immune cells and the supernate were collected for RT-PCR and ELISA detection related to RLH (RIG-1-like helicases) pathway. Results showed that the IL-4 and IFN-γ in supernate were increased after H1N1 infection, and the PA treatment suppressed the expression of cytokines and the mRNA of RLH pathway. PA anti-influenza virus may through regulate the RLH singal pathway.Graphical Abstract