Exogenous BDNF, NT-3 and NT-4 differentially regulate neurite outgrowth in cultured hippocampal neurons
Exogenous BDNF, NT-3 and NT-4 differentially regulate neurite outgrowth in cultured hippocampal neurons
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DOI：
10.1016/s0165-3806(00)00069-9
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发表时间：
2000-09-30
期刊：
影响因子：
--
通讯作者：
Leclerc, N
中科院分区：
文献类型：
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作者：
Labelle, C;Leclerc, N
多种生长因子有助于神经元树突和轴突过程的分化。培养的海马细胞按照明确的步骤精心制作树突和轴突过程。我们使用这个培养系统，以确定脑源性神经营养因子（BDNF），神经营养因子-3（NT-3）和神经营养因子-4（NT-4）的特定影响，在海马锥体神经元树突和轴突分化。我们证明了这些神经营养因子中的每一种对神经突生长产生不同的影响。BDNF和NT-3对未分化的神经突（称为小神经突）的生长和海马锥体神经元的轴突过程都有积极的影响。但NT-3的作用较BDNF更为重要。另一方面，NT-4并没有增强轴突的生长，但只对小神经突的生长有影响。由于细胞骨架蛋白在促进神经突生长中起着至关重要的作用，我们检测了与神经突生长相关的一些蛋白质的蛋白质水平：β-肌动蛋白，γ-肌动蛋白，α-微管蛋白，MAP 2和tau。令人惊讶的是，我们没有发现它们的蛋白质水平有任何变化。总之，我们的研究结果表明，BDNF，NT-3和NT-4发挥不同的影响，海马神经元的神经炎隔室。(C)2000 Elsevier Science B. V.保留所有权利。
Multiple growth factors contribute to the differentiation of dendritic and axonal processes by a neuron. Cultured hippocampal cells elaborate dendritic and axonal processes following well-defined steps. We used this culture system to determine the specific effects of brain-derived neurotrophic factor (BDNF), neurotrophin-3 (NT-3) and neurotrophin-4 (NT-4) on dendritic and axonal differentiation in hippocampal pyramidal neurons. We demonstrated that each of these neurotrophins exert distinct effects on neurite outgrowth. Both BDNF and NT-3 had positive effects on the outgrowth of undifferentiated neurites, called minor neurites, and on the axonal process of hippocampal pyramidal neurons. However, the effect of NT-3 was more important than that of BDNF. On the other hand, NT-4 did not enhance axonal outgrowth but had only an effect on the outgrowth of minor neurites. Since cytoskeletal proteins play crucial roles in promoting neurite outgrowth, we examined the protein levels of some of these proteins that are associated with neurite outgrowth: beta-actin, gamma-actin, alpha-tubulin, MAP2 and tau. Surprisingly, we did not detect any change in their protein levels. Taken together, our results show that BDNF, NT-3 and NT-4 exert distinct effects on the neuritic compartments of hippocampal neurons. (C) 2000 Elsevier Science B.V. All rights reserved.