PARTIAL CHARACTERIZATION, ORIGIN AND TURNOVER OF GLYCOPROTEINS OF THE RABBIT VITREOUS BODY
PARTIAL CHARACTERIZATION, ORIGIN AND TURNOVER OF GLYCOPROTEINS OF THE RABBIT VITREOUS BODY
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DOI：
10.1016/0014-4835(90)90065-3
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发表时间：
1990-08-01
影响因子：
3.4
通讯作者：
COSTA, MSA
中科院分区：
文献类型：
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作者：
HADDAD, A;LAICINE, EM;COSTA, MSA
将L-[3H]岩藻糖玻璃体内注射到兔子体内，注射后1小时至28天处死兔子。对玻璃体进行放射测量技术处理，然后进行电泳和荧光照相。未结合的[3H]岩藻糖在1天之内保持在高水平，而与糖蛋白结合的[3H]岩藻糖在注射后3天出现峰值，随后持续下降。玻璃体糖蛋白的周转率估计为每天4.37%，周转时间为22.85天。电泳和荧光成像结合显示了大约 14 个带有岩藻糖残基的糖蛋白条带，强烈表明各个糖蛋白之间的周转率存在差异。考马斯亮蓝染色凝胶中最显着的条带是分子量为 69 kDa 的条带，并且未用 [3H] 葡萄糖标记。该条带初步鉴定为血清白蛋白。另一方面，[3H]岩藻糖标记的14条带是源自眼睛内部的糖蛋白，也就是说，它们是玻璃体的固有成分。
L-[3H]fucose was injected intravitreally into rabbits that were killed from 1 hr to 28 days after injection. The vitreous bodies were processsed for radiometric techniques, and electrophoresis follwed by fluorgraphy. Unbound [3H]fucose remained at a high level up to 1 day, whereas the peak of [3H]fucose bound to glycoproteins was observed at 3 days after injection with a continuous decrease afterwards. The turnover rate of vitreous glycoproteins were estimated at 4.37% per day and their turnover time of 22.85 days. Electrophoresis and fluorgraphy combined revealed about 14 bands of glycoproteins with fucose residues and ther were strong indications of differences in turnover rate among individual glycoproteins. The most prominent band in the Coomassie blue-stained gels was the one having a molecular weight of 69 kDa and it was not labeled with [3H]gucose. This band was tentatively identified as serum albumin. On the other hand, the 14 bands labeled with [3H]fucose were glycoproteins originating from within the eye, that is, they are intrinsic constituents of the vitreous body.