Lnfluence of interleukin 10 and transforming growth factor-β on T cell stimulation through allogeneic retinal pigment epithelium cells in vitro
Lnfluence of interleukin 10 and transforming growth factor-β on T cell stimulation through allogeneic retinal pigment epithelium cells in vitro
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DOI：
10.1159/000063875
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发表时间：
2002-07-01
影响因子：
2.1
通讯作者：
Kohen, L
中科院分区：
文献类型：
--
作者：
Enzmann, V;Hollorn, M;Kohen, L
通过测量体外经视网膜色素上皮（RPE）细胞刺激后同种异体T细胞的增殖来测试白细胞介素10（IL-10）和转化生长因子-β（TGF-β）对人RPE细胞的免疫能力的影响。为此，使用不同浓度的干扰素-β（0、50、100和250 U/ml）作为刺激细胞激活人RPE细胞。用基于四唑的比色测定法在长达4天的时间内测量以下T细胞增殖。我们的研究结果表明，RPE细胞能够刺激同种异体T细胞的存在或不存在的专业抗原呈递细胞（巨噬细胞）。此外，IL-10或TGF-β可显著降低增殖。最大的抑制，特别是TGF-β，发现后48和72小时的孵育。这表明人RPE细胞的抗原呈递特性可以在培养物中改变。版权所有（C）2002 S. Karger AG，巴塞尔。
The effect of interleukin 10 (IL-10) and transforming growth factor-beta (TGF-beta) on the immunological capability of human retinal pigment epithelium (RPE) cells was tested by measuring the proliferation of allogeneic T cells after stimulation through the RPE cells in vitro. For this, human RPE cells were activated with different concentrations of interferon-beta (0, 50, 100 and 250 U/ml) used as stimulator cells. The following T cell proliferation was measured with a tetrazolium-based colorimetric assay over a period of up to 4 days. Our results show that RPE cells are able to stimulate allogeneic T cells in the presence or absence of professional antigen-presenting cells (macrophages). Additionally, the proliferation could be significantly decreased by IL-10 or TGF-beta. The maximum of suppression, especially by TGF-beta, was found after 48 and 72 h of incubation. This shows that the antigen-presenting properties of human RPE cells can be altered in culture. Copyright (C) 2002 S. Karger AG, Basel.