Is there replication-associated mutational pressure in the Saccharomyces cerevisiae genome?
Is there replication-associated mutational pressure in the Saccharomyces cerevisiae genome?
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DOI：
10.1006/jtbi.1999.1062
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发表时间：
2000-02-21
影响因子：
2
通讯作者：
Cebrat, S
中科院分区：
文献类型：
--
作者：
Gierlik, A;Kowalczuk, M;Cebrat, S
用去势DNA步行法分析酵母染色体的组成偏向。与真细菌染色体不同，酵母染色体没有表现出与复制起点和终点相关的特异性不对称。这可能是自主复制序列(ARS)相对过多以及在每个复制周期中随机选择这些序列的结果。然而，染色体两端的最后一个ARS负责与DNA链预先确定的领先/滞后作用的亚端粒序列的单向复制。在这些序列中观察到一种特殊的不对称性，类似于复制相关的突变压力引入到真细菌染色体中的不对称性。(C)2000年学术出版社。
Compositional bias of yeast chromosomes was analysed using detrended DNA walks. Unlike eubacterial chromosomes, the yeast chromosomes did not show the specific asymmetry correlated with origin and terminus of replication. It is probably a result of a relative excess of autonomously replicating sequences (ARS) and of random choice of these sequences in each replication cycle. Nevertheless, the last ARS from both ends of chromosomes are responsible for unidirectional replication of subtelomeric sequences with pre-established leading/lagging roles of DNA strands. In these sequences a specific asymmetry is observed, resembling the asymmetry introduced by replication-associated mutational pressure into eubacterial chromosomes. (C) 2000 Academic Press.