MicroRNA gga-miR-130b Suppresses Infectious Bursal Disease Virus Replication via Targeting of the Viral Genome and Cellular Suppressors of Cytokine Signaling 5
MicroRNA gga-miR-130b Suppresses Infectious Bursal Disease Virus Replication via Targeting of the Viral Genome and Cellular Suppressors of Cytokine Signaling 5
复制标题
gga-miR-130b 通过靶向病毒基因组和细胞 SOCS5 抑制传染性法氏囊病病毒复制
DOI：
10.1128/jvi.01646-17
复制
发表时间：
2018-01-01
影响因子：
5.4
通讯作者：
Zheng, Shijun J.
中科院分区：
文献类型：
--
作者：
Fu, Mengjiao;Wang, Bin;Zheng, Shijun J.
MicroRNAs(MiRNAs)是一种小的非编码RNA，通过沉默或降解靶标在转录后调节基因的表达，从而在免疫反应中发挥重要作用。然而，miRNAs在宿主对传染性法氏囊病病毒(IBDV)感染的应答中的作用尚不清楚。在本研究中，我们发现在感染IBDV后，DF-1细胞中一系列miRNAs的表达发生了显著变化。我们发现miRNA gga-miR-130b通过靶向IBDV A节段的特定序列而抑制IBDV的复制，并通过靶向宿主细胞中的细胞因子信号转导抑制物5(SOCS5)来增强β干扰素(IFN-β)的表达。这些发现表明gga-miR130b-3p在宿主对IBDV感染的防御中起着至关重要的作用。本工作表明gga-miR-130b通过直接靶向病毒基因组和细胞内I型干扰素表达的负调控因子SOCS5抑制IBDV复制，揭示了gga-miR-130抑制IBDV复制的机制。这些信息将有助于理解宿主细胞如何通过自身编码的小RNA来对抗病原性感染，并进一步了解microRNAs在细胞对病毒感染的反应中所起的作用。
MicroRNAs (miRNAs) are small noncoding RNAs that regulate gene expression posttranscriptionally through silencing or degrading their targets, thus playing important roles in the immune response. However, the role of miRNAs in the host response against infectious bursal disease virus (IBDV) infection is not clear. In this study, we show that the expression of a series of miRNAs was significantly altered in DF-1 cells after IBDV infection. We found that the miRNA gga-miR-130b in-hibited IBDV replication via targeting the specific sequence of IBDV segment A and enhanced the expression of beta interferon (IFN-beta) by targeting suppressors of cytokine signaling 5 (SOCS5) in host cells. These findings indicate that gga-miR130b-3p plays a crucial role in host defense against IBDV infection.IMPORTANCE This work shows that gga-miR-130b suppresses IBDV replication via directly targeting the viral genome and cellular SOCS5, the negative regulator for type I interferon expression, revealing the mechanism underlying gga-miR-130-induced inhibition of IBDV replication. This information will be helpful for the understanding of how host cells combat pathogenic infection by self-encoded small RNA and furthers our knowledge of the role of microRNAs in the cell response to viral infection.