Banana Transcription Factor MaERF11 Recruits Histone Deacetylase MaHDA1 and Represses the Expression of MaACO1 and Expansins during Fruit Ripening
Banana Transcription Factor MaERF11 Recruits Histone Deacetylase MaHDA1 and Represses the Expression of MaACO1 and Expansins during Fruit Ripening
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DOI：
10.1104/pp.16.00301
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发表时间：
2016-06-01
期刊：
影响因子：
7.4
通讯作者：
Lu, Wang-Jin
中科院分区：
文献类型：
--
作者：
Han, Yan-Chao;Kuang, Jian-Fei;Lu, Wang-Jin
植物激素乙烯通过调节乙烯信号途径控制果实成熟等多种发育和生理过程。我们的前期研究发现乙烯反应因子11（MaERF11）是乙烯信号通路中的一个转录因子，对香蕉的成熟具有负调控作用，但MaERF11介导的转录调控机制尚不清楚。在这里，我们发现MaERF11在植物中具有内在的转录抑制活性。电泳迁移率变动分析和染色质免疫沉淀分析表明，MaERF11结合到三个成熟相关的Expansin基因，MaEXP2，MaEXP7和MaEXP8的启动子，以及乙烯生物合成基因MaACO1，通过GCC盒基序。此外，MaACO1，MaEXP2，MaEXP7和MaEXP8基因的表达模式与果实成熟过程中组蛋白H3和H4乙酰化水平的变化相关。此外，我们发现MaERF11与具有组蛋白去乙酰化酶活性的组蛋白去乙酰化酶MaHDA1发生物理相互作用，这种相互作用显著增强了MaERF11介导的MaACO1和Expansins的转录抑制。总之，这些发现表明MaERF11可能通过组蛋白去乙酰化将MaHDA1募集到其靶基因并抑制其表达。
Phytohormone ethylene controls diverse developmental and physiological processes such as fruit ripening via modulation of ethylene signaling pathway. Our previous study identified that ETHYLENE RESPONSE FACTOR11 (MaERF11), a transcription factor in the ethylene signaling pathway, negatively regulates the ripening of banana, but the mechanism for the MaERF11-mediated transcriptional regulation remains largely unknown. Here we showed that MaERF11 has intrinsic transcriptional repression activity in planta. Electrophoretic mobility shift assay and chromatin immunoprecipitation analyses demonstrated that MaERF11 binds to promoters of three ripening-related Expansin genes, MaEXP2, MaEXP7 and MaEXP8, as well as an ethylene biosynthetic gene MaACO1, via the GCC-box motif. Furthermore, expression patterns of MaACO1, MaEXP2, MaEXP7, and MaEXP8 genes are correlated with the changes of histone H3 and H4 acetylation level during fruit ripening. Moreover, we found that MaERF11 physically interacts with a histone deacetylase, MaHDA1, which has histone deacetylase activity, and the interaction significantly strengthens the MaERF11-mediated transcriptional repression of MaACO1 and Expansins. Taken together, these findings suggest that MaERF11 may recruit MaHDA1 to its target genes and repress their expression via histone deacetylation.