Occurrence of red complex microorganisms and Aggregatibacter actinomycetemcomitans in patients with diabetes
Occurrence of red complex microorganisms and Aggregatibacter actinomycetemcomitans in patients with diabetes
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DOI：
10.1111/jicd.12051
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发表时间：
2015-02-01
影响因子：
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通讯作者：
Botero, Javier E.
中科院分区：
文献类型：
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作者：
Castrillon, Cesar A.;Hincapie, Juan P.;Botero, Javier E.
目的：本研究的目的是分析糖尿病患者中牙龈卟啉单胞菌（P. gingivalis）、连翘坦氏菌（T. forsythia）、齿垢密螺旋体（T. denticola）和伴放线聚集菌（A. actinomycetemcomitans）的发生情况。方法：从牙周和糖尿病参数以及龈下生物膜样本中获取60 名糖尿病患者和 62 名非糖尿病患者。通过使用聚合酶链反应，确定了红色复合微生物（牙龈卟啉单胞菌、连翘连翘和齿垢连翘）和伴放线放线菌的流行率。对组间进行描述性和非参数统计分析（Kruskal-Wallis、Mann-Whitney U 检验和 Fisher 精确检验）。结果：与非糖尿病患者相比，糖尿病患者的牙周附着丧失水平显着更高。糖尿病患者中检测到红色复合微生物的频率较低。与全身健康、无牙周炎的患者相比，患有糖尿病和牙周炎的患者中 A. actinomycetemcomitans 的检出率更高（P < 0.05）。 P. gingivalis 与非糖尿病患者的牙周炎相关（P < 0.05），而 A. actinomycetemcomitans 与糖尿病患者的牙周炎相关（P < 0.05）。结论：本研究的结果表明，糖尿病患者和非糖尿病患者的龈下微生物群可能存在差异。此外，牙龈卟啉单胞菌和伴放线放线菌分别与非糖尿病患者和糖尿病患者的牙周炎相关。
Aim: The aim of the present study was to analyze the occurrence of Porphyromonas gingivalis (P. gingivalis), Tannerella forsythia (T. forsythia), Treponema denticola (T. denticola), and Aggregatibacter actinomycetemcomitans (A. actinomycetemcomitans) in patients with diabetes.Methods: Periodontal and diabetic parameters and subgingival biofilm samples were obtained from 60 patients with diabetes and 62 patients without diabetes. By using polymerase chain reaction, the prevalence of red complex microorganisms (P. gingivalis, T. forsythia, and T. denticola) and A. actinomycetemcomitans were determined. Descriptive and non-parametric statistical analyses between groups were performed (Kruskal-Wallis, Mann-Whitney U-test, and Fisher's exact test).Results: Patients with diabetes presented significantly higher periodontal attachment loss levels compared to patients without diabetes. Red complex microorganisms were detected in lower frequencies in patients with diabetes. The detection of A. actinomycetemcomitans was higher in patients with diabetes and periodontitis compared to systemically-healthy patients without periodontitis (P < 0.05). P. gingivalis was associated with periodontitis in non-diabetic patients (P < 0.05), whereas A. actinomycetemcomitans was associated with periodontitis in diabetic patients (P < 0.05).Conclusions: The findings of the present study indicate that there might be differences in the subgingival microbiota between diabetic and non-diabetic patients. In addition, P. gingivalis and A. actinomycetemcomitans were associated with periodontitis in patients without diabetes and patients with diabetes, respectively.