Overexpression of B7-H3 in CD14+ monocytes is associated with renal cell carcinoma progression
Overexpression of B7-H3 in CD14+ monocytes is associated with renal cell carcinoma progression
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CD14 单核细胞中 B7-H3 的过度表达与肾细胞癌进展相关
DOI：
10.1007/s12032-014-0349-1
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发表时间：
2014-12-01
期刊：
影响因子：
3.4
通讯作者：
Hou, Jianquan
中科院分区：
文献类型：
--
作者：
Li, Miao;Zhang, Guangbo;Hou, Jianquan
肾细胞癌(RCC)是一种典型的多血管肿瘤，死亡率高。肿瘤诱导的血管生成对肿瘤的生长和转移至关重要。它在RCC的发展和进步中也起着重要的作用。然而，其分子机制仍不清楚。在本研究中，我们用流式细胞术检测了56例配对的肾癌组织和远端正常肾组织中CD14+单核细胞中B7-H3的表达水平，并用免疫组织化学方法定位了B7-H3和CD14的共表达。并通过卡方检验和Fisher精确检验分析其与临床病理特征的关系。结果表明，B7-H3和CD14在血管周围的肿瘤间质中共表达，肿瘤组织中B7-H3的表达水平高于正常组织(63.42±11.92vs.15.59±3.01，P<0.0001)。此外，其表达水平与肾癌分期(P=0.000)、淋巴结转移(P=0.003)、远处转移(P=0.020)、核分级(P=0.004)显著相关。综上所述，我们发现B7-H3和CD14在肾癌组织中共表达的现象。
Renal cell carcinoma (RCC) is well known as a typical hypervascular tumor and has a high mortality rate. Tumor-induced angiogenesis is crucial for tumor growth and metastasis. It also plays an important role in the development and progression of RCC. However, the molecular mechanism is still unclear. In our study, we evaluated the expression level of B7-H3 in CD14+ monocytes in 56 paired RCC samples and distant normal tissues by flow cytometry and located the co-expression of B7-H3 and CD14 by immunohistochemistry. In addition, we analyzed its association with clinical pathologic features through Chi-square test and Fisher exact test. Results showed that B7-H3 and CD14 co-expressed in tumor stroma surrounding the vessels and that the level of B7-H3 expression was higher in tumor than in normal tissues (63.42 ± 11.92 vs. 15.59 ± 3.01, P < 0.0001). Furthermore, the expression level was significantly associated with RCC stage (P = 0.000), nodal metastasis (P = 0.003), distant metastasis (P = 0.020), and nuclear grade (P = 0.004). Conclusively, we found the phenomenon that B7-H3 and CD14 co-expressed in RCC tissues.