Paeonol attenuates TNBS-induced colitis by inhibiting NF-κB and STAT1 transactivation
Paeonol attenuates TNBS-induced colitis by inhibiting NF-κB and STAT1 transactivation
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DOI：
10.1016/j.taap.2006.07.002
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发表时间：
2006-11-15
影响因子：
3.8
通讯作者：
Goto, Hidemi
中科院分区：
文献类型：
--
作者：
Ishiguro, Kazuhiro;Ando, Takafumi;Goto, Hidemi
丹皮酚是牡丹皮的主要酚类成分，具有抗炎作用。然而，丹皮酚对结肠炎的影响尚未得到评价，其抗炎作用的分子机制尚不清楚。本研究的目的是确定丹皮酚灌肠是否减轻三硝基苯磺酸（TNBS）诱导的小鼠结肠炎。我们还研究了丹皮酚对肿瘤坏死因子α (TNF α)和/或干扰素γ (IFN γ)治疗的结肠癌源性CW-2细胞和T细胞白血病源性Jurkat细胞的影响，这些细胞在tnbs诱导的结肠炎中起关键作用。从体重减轻、结肠长度和组织学评分来看，丹皮酚灌肠可减轻tnbs诱导的结肠炎。丹皮酚灌肠也降低了结肠髓过氧化物酶活性和诱导型一氧化氮合酶（iNOS）的产生。在CW-2细胞中，丹皮酚可抑制TNF α和IFN γ共刺激诱导的NOS蛋白和mRNA的表达。此外，丹皮酚还能降低CW-2细胞和Jurkat细胞中TNF α诱导的NF-kappa B反活化和ifn - γ诱导的STAT1反活化。这些发现提示丹皮酚灌肠可能对结肠炎的治疗有用。(c) 2006爱思唯尔公司版权所有。
Paeonol, a major phenolic component of Moutan Cortex, is known to have anti-inflammatory activity. However, the effect of Paeonol on colitis has not been evaluated and the molecular mechanism of its anti-inflammatory action remains unknown. The aim of this study was to determine if Paeonol enema attenuates trinitrobenzene sulfonic acid (TNBS)-induced colitis in mice. We also investigated the effects of Paeonol in colon cancer-derived CW-2 cells and T cell leukemia-derived Jurkat cells treated with tumor necrosis factor alpha (TNF alpha) and/or interferon gamma (IFN gamma), which play critical roles in TNBS-induced colitis. Paeonol enema attenuated TNBS-induced colitis judging by body weigh reduction, colon length and histological score. Myeloperoxidase activity and inducible nitric oxide synthase (iNOS) production in the colon were also reduced with Paeonol enema. In CW-2 cells, Paeonol inhibited NOS protein and mRNA expression induced by costimulation of TNF alpha and IFN gamma. Furthermore, Paeonol reduced TNF alpha-induced NF-kappa B transactivation and IFN-gamma-induced STAT1 transactivation in CW-2 cells and also in Jurkat cells. These findings suggest that Paeonol enema may be useful for the treatment of colitis. (c) 2006 Elsevier Inc. All rights reserved.