Species identification by analysis of bone collagen using matrix-assisted laser desorption/ionisation time-of-flight mass spectrometry
Species identification by analysis of bone collagen using matrix-assisted laser desorption/ionisation time-of-flight mass spectrometry
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DOI：
10.1002/rcm.4316
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发表时间：
2009-12-01
影响因子：
2
通讯作者：
Wilson, Julie C.
中科院分区：
文献类型：
--
作者：
Buckley, Michael;Collins, Matthew;Wilson, Julie C.
在没有明确形态学标记的情况下，对碎片骨的物种鉴定通常很困难，例如在肉类和骨粉中或考古遗址中。在这里，我们提出了一种通过质谱分析属特异性胶原蛋白肽的稳健方法，该方法简单地使用固相萃取（C18 ZipTip（R））进行肽纯化，而不是液相色谱/质谱（LC/MS）。对来自32种不同哺乳动物物种的胶原蛋白的分析确定了总共92种肽标记物，这些标记物可用于物种鉴定，例如，在加工食品和动物饲料中。一组古老的（>100 ka@10 ℃）的骨骼样本也进行了分析，以表明所提出的方法具有考古骨骼鉴定的应用。版权所有（C）2009约翰威利父子有限公司
Species identification of fragmentary bone, such as in rendered meat and bone meal or from archaeological sites, is often difficult in the absence of clear morphological markers. Here we present a robust method of analysing genus-specific collagen peptides by mass spectrometry simply by using solid-phase extraction (a C18 ZipTip (R)) for peptide purification, rather than liquid chromatography/mass spectrometry (LC/MS). Analysis of the collagen from 32 different mammal species identified a total of 92 peptide markers that could be used for species identification, for example, in processed food and animal feed. A set of ancient (>100 ka@10 degrees C) bone samples was also analysed to show that the proposed method has applications to archaeological bone identification. Copyright (C) 2009 John Wiley & Sons, Ltd.