A novel accessory role of neutrophils in concanavalin A-induced hepatitis
A novel accessory role of neutrophils in concanavalin A-induced hepatitis
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DOI：
10.1016/j.cellimm.2005.03.003
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发表时间：
2005-01-01
影响因子：
4.3
通讯作者：
Kobayashi, Y
中科院分区：
文献类型：
--
作者：
Hatada, S;Ohta, T;Kobayashi, Y
刀豆蛋白A(ConA)诱导的肝炎是一种T细胞介导的肝损伤模型，其中干扰素-γ通过诱导肝细胞的凋亡发挥重要作用。由于模型中有大量的中性粒细胞渗入肝脏，因此本研究探讨了中性粒细胞的作用。根据血浆丙氨酸氨基转移酶水平和组织化学的评估，刀豆蛋白A几乎没有引起中性粒细胞耗竭小鼠的肝损伤。中性粒细胞耗尽的小鼠也无法产生干扰素-γ。细胞内fFN-γ染色显示，在肝白细胞中，T细胞和NK细胞是产生干扰素-γ的主要细胞，而不是中性粒细胞。然而，尼龙羊毛纯化的“T细胞”在体外不能产生对ConA反应的干扰素-γ，而通过添加中性粒细胞可以恢复这种产生。总之，这项研究表明，在ConA诱导的肝炎中，中性粒细胞在干扰素-γ的产生中起着新的辅助作用。(C)2005 Elsevier Inc.保留所有权利。
Concanavalin A (Con A)-induced hepatitis has been investigated as a model of T cell-mediated liver injury, in which IFN-gamma plays an essential role by inducing apoptosis of liver cells. Since a large number of neutrophils infiltrate into the liver in the model, the role of neutrophils was investigated in this Study. Con A hardly caused liver injury in neutrophil-depleted mice, as assessed as to the plasma alanine aminotransferase level as well as histochemistry. Neutrophil-depleted mice also failed to produce IFN-gamma. IntracellUlar fFN-gamma staining revealed that, among liver leukocytes, T and NK cells but not neutrophils are the main producers of IFN-gamma. Nylon wool-purified "T cells," however, failed to produce IFN-gamma in response to Con A in vitro, while the production was restored by the addition of neutrophils. Overall, this study suggests that neutrophils play a novel accessory role in IFN-gamma production in Con A-induced hepatitis. (C) 2005 Elsevier Inc. All rights reserved.