Identification and Potential Application of Human Corneal Endothelial Progenitor Cells
Identification and Potential Application of Human Corneal Endothelial Progenitor Cells
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DOI：
10.1089/scd.2013.0387
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发表时间：
2014-09-15
影响因子：
4
通讯作者：
Nishida, Kohji
中科院分区：
文献类型：
--
作者：
Hara, Susumu;Hayashi, Ryuhei;Nishida, Kohji
角膜内皮被认为是由眼周间质经神经嵴发育而来的。人角膜内皮祖细胞（hcep）因其在组织再生医学中的潜在应用而受到广泛的研究。然而，HCEPs的存在及其性质尚未得到阐明。我们首先建立了一种新型的hcep无血清培养系统。hcep高表达p75神经营养因子受体、SOX9和FOXC2，部分保留了神经嵴和眼周间质的特性。此外，通过Ussing chamber系统和兔角膜移植，我们证实了HCEP具有较高的增殖能力，分化后的HCEP片具有角膜内皮功能。这些发现表明，利用一种特殊的培养系统可以选择性地从角膜内皮细胞中扩增出HCEPs，并将为角膜再生医学提供细胞片。
The corneal endothelium is believed to be developmentally originated from the periocular mesenchyme via the neural crest. Human corneal endothelial progenitor cells (HCEPs) have been investigated because of their potential availability for the tissue regenerative medicine. However, the existence and the properties of HCEPs have not been elucidated yet. We first established a novel serum-free culture system for HCEPs. The HCEPs highly expressed p75 neurotrophin receptor, SOX9, and FOXC2, and partially retained the properties of neural crest and periocular mesenchyme. Further, we demonstrated that HCEPs had a high proliferative potency, and the differentiated HCEP sheets had corneal endothelial function by using the Ussing chamber system and transplantation to the rabbit cornea. These findings suggest that the HCEPs can be selectively expanded from the corneal endothelium using a specific culture system and will provide cell sheets for corneal regenerative medicine.