TATA box-binding protein (TBP)-related factor 2 (TRF2), a third member of the TBP family
TATA box-binding protein (TBP)-related factor 2 (TRF2), a third member of the TBP family
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DOI：
10.1073/pnas.96.9.4791
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发表时间：
1999-04-27
影响因子：
11.1
通讯作者：
Tjian, R
中科院分区：
文献类型：
--
作者：
Rabenstein, MD;Zhou, S;Tjian, R
TATA盒结合蛋白（TBP）是真核细胞中RNA聚合酶II转录装置的重要组成部分。直到最近，人们认为一般的转录机制在很大程度上是不变的，并依赖于一个单一的TBP，而一个大的和不同的激活子和抑制子的集合主要负责赋予特异性的转录起始。然而，现在看来，“基础”转录机制也有助于通过组织特异性版本的TBP相关因子以及组织特异性TBP相关因子（TRF1）在果蝇中负责基因选择性，在这里，我们报告了TBP相关因子（TRF2）的克隆，该因子在人类，果蝇，秀丽隐杆线虫和其他后生动物中发现，像TRF1和TBP一样，TRF2结合转录因子IIA（TFIIA）和TFIIB，并且似乎是更大的蛋白质复合物的一部分，TRF2的一级氨基酸结构表明在推定的DNA结合结构域中存在分歧，并且毫不奇怪，它不能结合含有典型TATA盒的DNA。最重要的是，TRF2与果蝇染色体上不同于TBP或TRF1的基因座相关，因此它可能具有不同的启动子特异性并调节选定的基因子集。这些发现表明，后生动物已经进化出多种TBP，以适应在发育和细胞分化过程中基因和表达模式的大量增加。
The TATA box-binding protein (TBP) is an essential component of the RNA polymerase II transcription apparatus in eukaryotic cells. Until recently, it was thought that the general transcriptional machinery was largely invariant and relied on a single TBP, whereas a large and diverse collection of activators and repressors were primarily responsible for imparting specificity to transcription initiation. However, it now appears that the "basal" transcriptional machinery also contributes to specificity via tissue-specific versions of TBP-associated factors as well as a tissue specific TBP-related factor (TRF1) responsible for gene selectivity in Drosophila, Here we report the cloning of a TBP-related factor (TRF2) that is found in humans, Drosophila, Caenorhabditis elegans, and other metazoans, Like TRF1 and TBP, TRF2 binds transcription factor IIA (TFIIA) and TFIIB and appears to be part of a larger protein complex, TRF2's primary amino acid structure suggests divergence in the putative DNA binding domain, and not surprisingly, it fails to bind to DNA containing canonical TATA boxes. Most importantly, TRF2 is associated with loci on Drosophila chromosomes distinct from either TBP or TRF1, so it may have different promoter specificity and regulate a select subset of genes. These findings suggest that metazoans have evolved multiple TBPs to accommodate the vast increase in genes and expression patterns during development and cellular differentiation.