EXPRESSION OF C-FOS MESSENGER-RNA FOLLOWING SEIZURES EVOKED FROM AN EPILEPTOGENIC SITE IN THE DEEP PREPIRIFORM CORTEX - REGIONAL DISTRIBUTION IN BRAIN AS SHOWN BY INSITU HYBRIDIZATION
EXPRESSION OF C-FOS MESSENGER-RNA FOLLOWING SEIZURES EVOKED FROM AN EPILEPTOGENIC SITE IN THE DEEP PREPIRIFORM CORTEX - REGIONAL DISTRIBUTION IN BRAIN AS SHOWN BY INSITU HYBRIDIZATION
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DOI：
10.1006/exnr.1993.1002
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发表时间：
1993-01-01
影响因子：
5.3
通讯作者：
GALE, K
中科院分区：
文献类型：
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作者：
MAGGIO, R;LANAUD, P;GALE, K
使用原位杂交组织化学检测c-fosmRNA，我们研究了激活的途径引起的癫痫发作的局部应用荷包牡丹碱到一个高度离散的致痫位点在梨状前皮质深部，该地区tempestas（AT）。30分钟后，开始由荷包牡丹碱引起的边缘运动癫痫发作在AT中，在海马结构，杏仁核，嗅球，梨状皮质和内嗅皮质中检测到c-fosmRNA的显着增加。AT中c-fosmRNA的增加严格依赖于药物的输注。在AT外输注相同剂量的荷包牡丹碱不会增加c-fosmRNA水平。c-fosmRNA表达的映射模式特异于边缘系统癫痫发作的程度通过检查另一种局灶性诱发癫痫发作模型进行评估，该模型涉及将荷包牡丹碱双侧应用于下丘。在由注射到下丘的荷包牡丹碱诱发的爆炸性奔跑-弹跳阵挛性癫痫发作后，边缘系统中没有任何可检测到的c-fosinduction，这表明我们在AT诱发的癫痫发作模型中发现的激活模式并不常见于所有形式的惊厥活动。此外，这些观察结果表明，我们所观察到的激活模式是癫痫依赖性的，而不是应激诱导的。我们的研究结果表明，c-fosmRNA的表达是有用的癫痫发作传播和激活的解剖模式是选择性相关的癫痫发作诱发的类型参与的途径的功能映射。
Usingin situhybridization histochemistry for the detection of c-fosmRNA, we examined the pathways activated by seizures evoked by a focal application of bicuculline into a highly discrete epileptogenic site in the deep prepiriform cortex, the area tempestas (AT). Thirty minutes after the initiation of limbic motor seizures evoked by bicuculline in AT, a marked increase in c-fosmRNA was detected in the hippocampal formation, amygdala, olfactory bulb, piriform cortex, and entorhinal cortex. The increase of c-fosmRNA was strictly dependent upon the infusion of the drug in AT. Infusions of bicuculline in the same dose outside the AT did not increase c-fosmRNA levels. The extent to which the mapping pattern of c-fosmRNA expression was specific to limbic seizures was evaluated by examining another focally evoked seizure model involving the application of bicuculline bilaterally into the inferior colliculus. The absence of any detectable c-fosinduction in the limbic system after explosive running-bouncing clonic seizures evoked by bicuculline injected into the inferior colliculus indicates that the pattern of activation that we found in the AT-evoked seizure model is not common to all forms of convulsive activity. Furthermore these observations suggest that the pattern of activation we have observed is seizure dependent and not stress induced. Our results indicate that c-fosmRNA expression is useful in the functional mapping of pathways involved in seizure propagation and that the anatomic pattern of activation is selectively related to the type of seizure evoked.