Visualization of DNA methylation and histone modifications in living cells
Visualization of DNA methylation and histone modifications in living cells
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DOI：
10.1016/j.ceb.2010.02.004
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发表时间：
2010-06-01
影响因子：
7.5
通讯作者：
Yamagata, Kazuo
中科院分区：
文献类型：
--
作者：
Kimura, Hiroshi;Hayashi-Takanaka, Yoko;Yamagata, Kazuo
DNA甲基化和组蛋白修饰在基因组功能中发挥着重要作用，包括表观遗传基因调控。当细胞核在发育和分化过程中被重新编程时，这些修饰会发生剧烈的变化。最近的研究使得在活的培养细胞和小鼠植入前胚胎中检测到修饰的动力学成为可能。DNA甲基化利用人MBD1蛋白的甲基-CpG结合域进行可视化。组蛋白修饰的水平和分布可以通过两种不同的方法进行监测。一种方法使用基于荧光/福斯特共振能量转移(FRET)的传感器，另一种方法使用特定抗体的荧光标记抗原结合片段。这些可视化技术将促进未来与发育、分化和疾病相关的表观遗传调控的研究。
DNA methylation and histone modifications play important roles in genome function, including epigenetic gene regulation. These modifications undergo drastic changes when nuclei are reprogrammed during development and differentiation. Recent studies have enabled the detection of the dynamics of modifications in living cultured cells and mouse preimplantation embryos. DNA methylation was visualized using the methyl-CpG-binding domain of the human MBD1 protein. The level and distribution of histone modifications can be monitored by two different methods. One approach uses fluorescence/Forster resonance energy transfer (FRET)-based sensors and another uses fluorescently labeled antigen binding fragments of specific antibodies. These visualization techniques will facilitate future studies on epigenetic regulation related to development, differentiation, and disease.