IMMUNOSUPPRESSIVE EFFECTS OF AFLATOXIN IN GROWING RATS
IMMUNOSUPPRESSIVE EFFECTS OF AFLATOXIN IN GROWING RATS
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DOI：
10.1007/bf01103737
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发表时间：
1993-12-01
期刊：
影响因子：
5.5
通讯作者：
RAY, PK
中科院分区：
文献类型：
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作者：
RAISUDDIN, S;SINGH, KP;RAY, PK
本实验研究了黄曲霉的致癌代谢产物黄曲霉毒素B-1（AFB（1））对生长期大鼠的免疫抑制作用。将断乳大鼠隔日经口给予60、300和600 μ g AFB（1）/kg体重亚慢性暴露4周。在对照组和给药组动物中评估了细胞介导免疫（CMI）和体液免疫的各种参数。通过测量迟发型超敏反应（DTH）反应和通过空斑形成细胞（PFC）测定来评估CMI。还进行了T细胞和B细胞的淋巴增殖反应试验。观察到黄曲霉毒素B（1）选择性抑制生长期大鼠的细胞免疫。通过DTH反应测定，AFB（1）仅在300和600 μ g剂量水平下抑制CMI。结论：生长期宿主持续低水平接触黄曲霉毒素可增强其对感染和肿瘤发生的易感性。
The immunosuppressive potential of aflatoxin B-1 (AFB(1)), the carcinogenic metabolite of Aspergillus flavus, was evaluated in growing rats. The weanling rats were subchronically exposed to 60, 300 and 600 mu g AFB(1)/kg body weight for four weeks on alternate days by oral feeding. Various parameters of cell mediated immunity (CMI) and humoral immunity were assessed in control and treated animals. CMI was evaluated by measuring delayed type of hypersensitivity (DTH) response and humoral by plaque forming cell (PFC) assay. The lymphoproliferative response assay for T- and B-cells was also performed. It was observed that AFB(1) selectively suppressed cell mediated immunity in growing rats. AFB(1) suppressed CMI at the 300 and 600 mu g dose levels only as measured by DTH response assay. It is concluded that continuous low level exposure of aflatoxin to growing host may enhance its susceptibility to infection and tumorigenesis.