Divergent human remodeling complexes remove nucleosomes from strong positioning sequences.
Divergent human remodeling complexes remove nucleosomes from strong positioning sequences.
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人体重塑配合物的发散复合物从强定位序列中去除核小体。
DOI：
10.1093/nar/gkp1030
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发表时间：
2010-01
影响因子：
14.9
通讯作者：
Schnitzler GR
中科院分区：
文献类型：
--
作者：
Pham CD;He X;Schnitzler GR
核小体定位在控制DNA对转录因子和其他核过程的可及性方面起着重要作用。组装后的核小体位置至少部分由DNA序列对组蛋白八聚体的相对亲和力决定。然而，核小体可以通过一类ATP依赖性染色质重塑复合物移动。我们最近发现，人类SWI/SNF重塑复合物以序列特异性方式移动核小体，远离核小体定位序列（NPSes）。在这里，我们比较了不同的生物功能（hSWI/SNF，SNF 2 h ATP酶和hACF，CHRAC和WICH复合物，每个复合物都含有SNF 2 h）在5S rDNA，MMTV和601个多核小体模板上的重新定位特异性。我们发现，所有五个重塑的行为类似，以减少核小体占用超过最强的NPSes，这种效果可能直接有助于WICH激活5S rDNA转录的功能。虽然在复合物之间观察到一些差异，但发现所有五种重塑剂导致令人惊讶地相似的核小体分布。这表明重塑复合物可能具有保守的重新定位特异性，并且它们不同的生物学功能可能主要来自复合物特异性亚基赋予的其他性质。
Nucleosome positioning plays a major role in controlling the accessibility of DNA to transcription factors and other nuclear processes. Nucleosome positions after assembly are at least partially determined by the relative affinity of DNA sequences for the histone octamer. Nucleosomes can be moved, however, by a class of ATP dependent chromatin remodeling complexes. We recently showed that the human SWI/SNF remodeling complex moves nucleosomes in a sequence specific manner, away from nucleosome positioning sequences (NPSes). Here, we compare the repositioning specificity of five remodelers of diverse biological functions (hSWI/SNF, the SNF2h ATPase and the hACF, CHRAC and WICH complexes than each contain SNF2h) on 5S rDNA, MMTV and 601 NPS polynucleosomal templates. We find that all five remodelers act similarly to reduce nucleosome occupancy over the strongest NPSes, an effect that could directly contribute to the function of WICH in activating 5S rDNA transcription. While some differences were observed between complexes, all five remodelers were found to result in surprisingly similar nucleosome distributions. This suggests that remodeling complexes may share a conserved repositioning specificity, and that their divergent biological functions may largely arise from other properties conferred by complex-specific subunits.
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影响因子：
5.6
作者：
Lowary, PT;Widom, J
通讯作者：
Widom, J
影响因子：
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作者：
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通讯作者：
Chakravarti, Debabrata
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作者：
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HAGER, GL
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作者：
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作者：
Fryer, CJ;Kinyamu, HK;Archer, TK
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Archer, TK