Neurons expressing trace amine-associated receptors project to discrete glomeruli and constitute an olfactory subsystem
Neurons expressing trace amine-associated receptors project to discrete glomeruli and constitute an olfactory subsystem
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DOI：
10.1073/pnas.1206724109
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发表时间：
2012-08-14
影响因子：
11.1
通讯作者：
Barnea, Gilad
中科院分区：
文献类型：
--
作者：
Johnson, Mark A.;Tsai, Lulu;Barnea, Gilad
痕量胺相关受体（TAAR）家族的一些化学感受器检测先天厌恶的气味，并被提议激活硬连线嗅觉回路。然而，TAAR神经元的布线，Taar基因选择的调节机制，以及TAAR蛋白的亚细胞定位仍然未知。在这里，我们揭示了表达TAAR和气味受体（OR）的神经元之间的相似性，但也有意想不到的差异。像OR一样，TAAR似乎在纤毛（气味检测的位点）和轴突（它们可能参与指导）中单等位基因表达和定位。TAAR神经元投射到离散的肾小球，主要局限于一个局限的球区域。Taar表达涉及与OR表达不同的调控逻辑，因为选择Taar5敲除等位基因的神经元经常表达第二个Taar而不沉默缺失的等位基因。此外，OR基因选择的表观遗传标记在Taar基因中不存在。TAAR神经元独特的分子和解剖学特征表明它们构成了一个独特的嗅觉子系统。
Some chemoreceptors of the trace amine-associated receptor (TAAR) family detect innately aversive odors and are proposed to activate hardwired olfactory circuits. However, the wiring of TAAR neurons, the regulatory mechanisms of Taar gene choice, and the subcellular localization of TAAR proteins remain unknown. Here, we reveal similarities between neurons expressing TAARs and odorant receptors (ORs), but also unexpected differences. Like ORs, TAARs seem to be monoallelically expressed and localized both in cilia, the site of odor detection, and in axons, where they may participate in guidance. TAAR neurons project to discrete glomeruli predominantly localized to a confined bulb region. Taar expression involves different regulatory logic than OR expression, as neurons choosing a Taar5 knockout allele frequently express a second Taar without silencing the deleted allele. Moreover, the epigenetic signature of OR gene choice is absent from Taar genes. The unique molecular and anatomical features of the TAAR neurons suggest that they constitute a distinct olfactory subsystem.