Ultrasound-mediated nanobubble destruction (UMND) facilitates the delivery of VEGFR2-targeted CD-TK-loaded cationic nanobubbles in the treatment of bladder cancer
Ultrasound-mediated nanobubble destruction (UMND) facilitates the delivery of VEGFR2-targeted CD-TK-loaded cationic nanobubbles in the treatment of bladder cancer
复制标题
超声介导的纳米泡破坏（UMND）促进VEGFR 2靶向CD-TK负载的阳离子纳米泡在膀胱癌治疗中的递送
DOI：
10.1007/s00432-020-03160-7
复制
发表时间：
2020-03-16
影响因子：
3.6
通讯作者：
Zhang, Ronggui
中科院分区：
文献类型：
--
作者：
Hu, Cong;Jiang, Depeng;Zhang, Ronggui
背景CD-TK双自杀基因已成为治疗膀胱癌的有效手段。一种新的分子靶向超声（US）方法已经开发出来，可以精确地将载有这种基因的纳米气泡引导到膀胱肿瘤细胞内的区域，并且由于其将药物递送到靶肿瘤的效率而被广泛使用。方法采用薄膜水合超声法、碳二亚胺化学法和静电吸附法制备负载CD-TK双自杀基因的均匀纳米气泡。结果本研究成功制备了负载CD-TK双自杀基因的阳离子纳米气泡，并与抗VEGFR 2抗体偶联，可与VEGFR 2阳性细胞结合。荧光和流式细胞术证据表明，成功开发了负载CD-TK双自杀基因的纳米气泡。通过超声介导的纳米泡破坏（UMND）递送的CD-TK-CNB增强了转染效率、CD-TK双自杀基因的过表达和肿瘤细胞凋亡，并在体外抑制肿瘤细胞生长。结论CD-TK-CNB有望成为治疗膀胱癌的新方法。
Background The CD-TK double suicide gene has become an effective therapy for bladder cancer. A novel molecular-targeted ultrasound (US) method has been developed to precisely guide nanobubbles loaded with this gene to regions within bladder tumor cells and is widely used due to its efficiency in delivering drugs to the target tumor. Methods Uniform nanoscaled nanobubbles loaded with CD-TK double suicide gene were developed using a thin-film hydration sonication, carbodiimide chemistry approaches, and electrostatic adsorption methods. Results In the present study, we synthesized CD-TK double suicide gene-loaded cationic nanobubbles conjugated with anti-VEGFR2 that can bind with VEGFR2-positive cells. Fluorescence and flow cytometry evidence show that CD-TK double suicide gene-loaded nanobubbles were successfully developed. CD-TK-CNBs delivered via US-mediated nanobubble destruction (UMND) enhanced transfection efficiency, overexpression of CD-TK double suicide gene, and tumor cell apoptosis, and inhibited tumor cell growth in vitro. Conclusions These CD-TK-CNBs may become a novel treatment for bladder cancer.