Formation of Direction-Controllable Pseudorotaxane and Catenane Using Chemically Cyclized Oligodeoxynucleotides and Their Noncovalent RNA Labeling
Formation of Direction-Controllable Pseudorotaxane and Catenane Using Chemically Cyclized Oligodeoxynucleotides and Their Noncovalent RNA Labeling
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DOI：
10.1021/acs.bioconjchem.3c00031
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发表时间：
2023-03-17
影响因子：
4.7
通讯作者：
Onizuka，Kazumitsu
中科院分区：
文献类型：
--
作者：
Kuwahara，Kazuki;Yajima，Sayaka;Onizuka，Kazumitsu
互锁结构的形成，如轮烷和索烃，使得非共价缀合成为可能。我们以前证实，化学环化的伪轮烷形成的寡脱氧核苷酸（prfODNs）与双尾部分通过滑动过程与靶DNA和RNA形成伪轮烷结构。在这里，我们报告的一步合成环化的prfODNs从炔修饰的ODNs，之后，我们研究了滑动过程的性质和机制，并进行非共价RNA标记与prfODNs。此外，链烷结构是通过与5′-末端磷酸化的RNA和酶促连接结合形成的假轮烷形成。新合成的prfODN代表了实现各种功能部分与RNA的非共价缀合的新工具。
The formation of interlocked structures, such as rotaxane and catenane, enables noncovalent conjugations. We previously confirmed that the chemically cyclized pseudorotaxane-forming oligodeoxynucleotides (prfODNs) with double-tailed parts formed a pseudorotaxane structure with the target DNA and RNA via the slipping process. Here, we report the one-step synthesis of cyclized prfODNs from alkyne-modified ODNs, after which we investigated the properties and mechanism of the slipping process and performed noncovalent RNA labeling with prfODNs. Additionally, the catenane structure was formed by the combination of pseudorotaxane formation with a 5′-end-phosphorylated RNA and enzymatic ligation. The newly synthesized prfODN represents a new tool for achieving the noncovalent conjugation of various functional moieties to RNAs.