Controlling complexity and water penetration in functional de novo protein design.
Controlling complexity and water penetration in functional de novo protein design.
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DOI：
10.1042/bst0361106
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发表时间：
2008-12
影响因子：
3.9
通讯作者：
Dutton PL
中科院分区：
文献类型：
--
作者：
Anderson JL;Koder RL;Moser CC;Dutton PL
天然蛋白质很复杂，支持功能和催化的工程元件也很模糊。简化的合成蛋白质支架提供了一种避免这种复杂性的方法，了解功能组装背后的基本原理，并使酶功能的模块化组装成为有形的现实。这种蛋白质设计的一个关键特征是控制和排除水进入蛋白质核心，以提供实现酶促功能的低介电环境。最近在从头蛋白质设计方面取得的成功说明了如何将这种控制纳入设计过程，并为这些系统中新生酶活性的合成铺平了道路。
Natural proteins are complex, and the engineering elements that support function and catalysis are obscure. Simplified synthetic protein scaffolds offer a means to avoid such complexity, learn the underlying principles behind the assembly of function and render the modular assembly of enzymatic function a tangible reality. A key feature of such protein design is the control and exclusion of water access to the protein core to provide the low-dielectric environment that enables enzymatic function. Recent successes in de novo protein design have illustrated how such control can be incorporated into the design process and have paved the way for the synthesis of nascent enzymatic activity in these systems.