Streptococcus suis II immunoassay based on thorny gold nanoparticles and surface enhanced Raman scattering
Streptococcus suis II immunoassay based on thorny gold nanoparticles and surface enhanced Raman scattering
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基于刺金纳米颗粒和表面增强拉曼散射的猪链球菌 II 免疫分析
DOI：
10.1039/c2an15997j
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发表时间：
2012-01-01
期刊：
影响因子：
4.2
通讯作者：
Luo, Zhihui
中科院分区：
文献类型：
--
作者：
Chen, Kun;Han, Heyou;Luo, Zhihui
以多刺金纳米粒子（tAuNPs）为表面增强拉曼散射（Sers）底物，建立了检测猪链球菌II型（SS 2）溶菌酶释放蛋白（MRP）抗体的免疫分析方法。首先，在没有模板和表面活性剂的情况下，通过种子介导的生长方法制备具有多个分支的tAuNPs，促进对巯基苯甲酸（pMBA）通过S-Au键共价缀合到tAuNPs上。所获得的提供强拉曼信号的免疫SERS标签使得有可能建立利用夹心测定法以高灵敏度水平间接检测针对SS 2的MRP抗体的应用。1588 cm（-1）处的拉曼强度与MRP抗体浓度的对数在10 pg mL（-1）至0.1 μ g mL（-1）的范围内成正比。通过使用免疫SERS标签，检测灵敏度显着提高到0.1 pg mL（-1）。将该方法应用于猪血清中MRP抗体的检测，结果与ELISA方法一致，表明该方法在临床诊断免疫分析中具有很好的应用前景。
An immunoassay based on surface enhanced Raman scattering (SERS) spectroscopy was developed to detect muramidase released protein (MRP) antibody against Streptococcus suis II (SS2) utilizing thorny gold nanoparticles (tAuNPs) as SERS substrates. Initially, tAuNPs with multi-branches were prepared by the seed-mediated growth method in the absence of templates and surfactants, facilitating p-mercaptobenzoic acid (pMBA) conjugation covalently onto the tAuNPs through S-Au bonds. The obtained immuno-SERS tag affording strong Raman signals made it possible to establish an application of indirect detection of the MRP antibody against SS2 with a sandwich assay at a highly sensitive level. The Raman intensity at 1588 cm(-1) was proportional to the logarithm of the concentration of MRP antibody in the range of 10 pg mL(-1) to 0.1 mu g mL(-1). The detection sensitivity was significantly improved to 0.1 pg mL(-1) by using the immuno-SERS tags. Furthermore, the proposed SERS approach was applied to detect MRP antibody in pig serum samples, and the results agreed well with those of ELISA, indicating great potential for clinical application in diagnostic immunoassays.