Role of Periostin in Adhesion and Migration of Bone Remodeling Cells.
Role of Periostin in Adhesion and Migration of Bone Remodeling Cells.
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DOI：
10.1371/journal.pone.0147837
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发表时间：
2016
期刊：
影响因子：
3.7
通讯作者：
Obaya AJ
中科院分区：
文献类型：
--
作者：
Cobo T;Viloria CG;Solares L;Fontanil T;González-Chamorro E;De Carlos F;Cobo J;Cal S;Obaya AJ
骨膜蛋白是一种细胞外基质蛋白，在受到持续机械应力的富含胶原的组织中高度表达。在功能上，骨膜蛋白参与组织重塑，其改变的功能与许多病理过程相关。骨膜蛋白在牙齿组织的维护中起着关键作用，并且它主要在发生张力或压力的那些区域中表达。在这方面，骨膜蛋白的高表达是必不可少的，以促进牙周膜成纤维细胞的迁移和增殖。然而，关于骨膜蛋白参与骨重建细胞迁移和粘附过程的研究却知之甚少。在这项工作中，我们采用小鼠前成骨细胞MC 3 T3-E1和巨噬细胞样RAW 264.7细胞系过表达骨膜蛋白，并进行不同的细胞为基础的测定，以研究细胞行为的变化。我们的数据表明，骨膜蛋白过表达不仅增加了MC 3 T3-E1细胞对不同基质蛋白的粘附能力，而且阻碍了它们的迁移能力。还分析了骨膜蛋白过表达后MC 3 T3-E1细胞RNA表达谱的变化，突出了与细胞迁移、粘附或骨代谢等过程有关但与骨分化无关的基因的改变。总的来说，我们的工作提供了新的证据骨膜蛋白在成骨细胞生理的影响。
Periostin is an extracellular matrix protein highly expressed in collagen-rich tissues subjected to continuous mechanical stress. Functionally, periostin is involved in tissue remodeling and its altered function is associated to numerous pathological processes. In orthodontics, periostin plays key roles in the maintenance of dental tissues and it is mainly expressed in those areas where tension or pressing forces are taking place. In this regard, high expression of periostin is essential to promote migration and proliferation of periodontal ligament fibroblasts. However little is known about the participation of periostin in migration and adhesion processes of bone remodeling cells. In this work we employ the mouse pre-osteoblastic MC3T3-E1 and the macrophage-like RAW 264.7 cell lines to overexpress periostin and perform different cell-based assays to study changes in cell behavior. Our data indicate that periostin overexpression not only increases adhesion capacity of MC3T3-E1 cells to different matrix proteins but also hampers their migratory capacity. Changes on RNA expression profile of MC3T3-E1 cells upon periostin overexpression have been also analyzed, highlighting the alteration of genes implicated in processes such as cell migration, adhesion or bone metabolism but not in bone differentiation. Overall, our work provides new evidence on the impact of periostin in osteoblasts physiology.