SEM characterization of a cellular layer separating blood vessels from endoderm in the quail embryo.
SEM characterization of a cellular layer separating blood vessels from endoderm in the quail embryo.
复制标题
鹌鹑胚胎中将血管与内胚层分开的细胞层的 SEM 表征。
DOI：
10.1002/ar.1092250212
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发表时间：
1989
期刊：
影响因子：
--
通讯作者：
Noden,DM
中科院分区：
文献类型：
--
作者：
Reiss,KZ;Noden,DM
用扫描电镜观察了9-11期鹌鹑胚胎内胚层和内脏中胚层与血管发育的关系。将胚胎腹侧朝上固定在琼脂平板上，并在固定和脱水前通过手术去除内胚层。这个过程暴露了一个网状的细胞层，紧密贴附在近轴中胚层的腹面和所有可见的血管上，我们称之为亚血管层。这一层的发育从吻侧到尾侧，从外侧到内侧，在节段性板的不分节的近轴中胚层上可以看到最早的形成阶段。血管下层密度明显增加，略位于胚内血管丛最内边界的内侧。这一层的细胞最终在侧板、近轴中胚层和脊索下形成连续的片层。随着成熟，血管下层的细胞接近汇合。胚胎维管组织和亚维管层的发育的空间和时间模式密切相关，表明亚维管层在正常胚胎维管发育中可能起作用。
The associations between the developing blood vessels and both endoderm and splanchnic mesoderm in quail embryos at stages 9–11 were examined by using scanning electron microscopy. Embryos were pinned ventral‐side up on agar plates and the endoderm was surgically removed prior to fixation and dehydration. This procedure exposes a netlike layer of cells closely apposed to the ventral surface of paraxial mesoderm and all visible blood vessels; we are calling this thesubvascular layer. Development of this layer proceeds rostral‐to‐caudal, and lateral‐to‐medial, with the earliest stages of formation being visible over the unsegmented paraxial mesoderm of the segmental plate. The subvascular layer increases markedly in density slightly medial to the innermost boundary of the intraembryonic vascular plexus. Cells of this layer eventually establish a continuous sheet beneath the lateral plate and paraxial mesoderm and the notochord. With maturation, the cells of the subvascular layer approach confluence. The spatial and temporal patterns of development of the embryonic vascular tissues and the subvascular layer are closely correlated, suggesting a possible role for the subvascular layer in normal embryonic vascular development.