Gene Expression Profile of Olfactory Transduction Signaling in an Animal Model of Human Multiple Sclerosis
Gene Expression Profile of Olfactory Transduction Signaling in an Animal Model of Human Multiple Sclerosis
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DOI：
10.5607/en.2019.28.1.74
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发表时间：
2019-02-01
影响因子：
2.4
通讯作者：
Shin, Taekyun
中科院分区：
文献类型：
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作者：
Kim, Jeongtae;Ahn, Meejung;Shin, Taekyun
嗅觉功能障碍发生在人类多发性硬化症以及实验性自身免疫性脑脊髓炎（EAE）动物模型中。本研究的目的是通过下一代测序分析受 EAE 影响的小鼠嗅球中的差异表达基因 (DEG)，特别关注嗅觉相关信号的变化。使用髓磷脂少突胶质细胞糖蛋白和佐剂免疫后，C57BL/6 小鼠中诱导 EAE。在免疫小鼠的嗅球和脊髓中发现了炎症病变。对受 EAE 影响的小鼠嗅球中的 DEG 进行分析发现，44 个基因上调（主要与炎症介质相关），而 519 个基因下调；其中，与嗅觉信号转导相关的嗅觉标记蛋白和stomatin-like 3显着下调（log2 [倍数变化] > 1且p值
Olfactory dysfunction occurs in multiple sclerosis in humans, as well as in an animal model of experimental autoimmune encephalomyelitis (EAE). The aim of this study was to analyze differentially expressed genes (DEGs) in olfactory bulb of EAE-affected mice by next generation sequencing, with a particular focus on changes in olfaction-related signals. EAE was induced in C57BL/6 mice following immunization with myelin oligodendrocyte glycoprotein and adjuvant. Inflammatory lesions were identified in the olfactory bulbs as well as in the spinal cord of immunized mice. Analysis of DEGs in the olfactory bulb of EAE-affected mice revealed that 44 genes were upregulated (and which were primarily related to inflammatory mediators), while 519 genes were downregulated; among the latter, olfactory marker protein and stomatin-like 3, which have been linked to olfactory signal transduction, were significantly downregulated (log2 [fold change] > 1 and p-value