Clinical significance of kallikrein-related peptidase (KLK10) mRNA expression in colorectal cancer
Clinical significance of kallikrein-related peptidase (KLK10) mRNA expression in colorectal cancer
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DOI：
10.1016/j.clinbiochem.2013.03.002
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发表时间：
2013-10-01
影响因子：
2.8
通讯作者：
Scorilas, Andreas
中科院分区：
文献类型：
--
作者：
Alexopoulou, Dimitra K.;Papadopoulos, Iordanis N.;Scorilas, Andreas
目的：结直肠癌（Colorectal cancer，CRC）是男性和女性最常见的三大恶性肿瘤之一。尽管有几种生物标志物用于疾病的诊断和预后，但它们的特异性和灵敏度有限。人激肽释放酶相关肽酶10（KLK 10）基因是人组织激肽释放酶家族的成员。由于前列腺特异性抗原（PSA）是检测和监测前列腺癌的最佳生物标志物，是该家族的成员，因此包括KLK 10在内的许多其他成员已被广泛研究为不同癌症类型的新型生物标志物。在以前的研究中，KLK 10已被提出作为卵巢癌的诊断生物标志物，而其外显子3的甲基化已被提出作为早期乳腺癌患者的预后标志物。本研究的目的是分析KLK 10 mRNA的表达，并研究其预后价值和潜在的临床应用作为一种新的分子组织生物标志物在CRC.Design和Methods：研究组包括190个结直肠样本。从粉碎的组织中提取总RNA，并通过逆转录制备cDNA。通过实时PCR扩增KLK 10。结果：KLK 10在结肠癌组织中的表达明显高于正常结肠组织（P < 0.001）;晚期TNM和Dukes'分期的肿瘤中KLK 10表达水平较高（P = 0.036; P = 0.025）。KLK 10高表达组患者的无瘤生存率和总生存率较低（P = 0.014; P = 0.020）。结论：KLK 10可能成为结直肠癌预后不良的新指标。(c)2013年加拿大临床化学家协会。爱思唯尔公司出版All rights reserved.
Objectives: Colorectal cancer (CRC) is one of the three most common cancers in both genders. Even though several biomarkers are in use in diagnosis and prognosis of the disease, they are marred by limited specificity and sensitivity. The human kallikrein-related peptidase 10 (KLK10) gene is a member of the human tissue kallikrein family. Because prostate specific antigen (PSA), the best biomarker for detecting and monitoring prostate cancer, is a member of this family, many other members, including KLK10, have been widely examined as novel biomarkers for different cancer types. In previous studies, KLK10 has been proposed as a diagnostic biomarker for ovarian carcinoma, while its methylation on exon 3 has been proposed as a prognostic marker for early-stage breast cancer patients. The purpose of this study was to analyse KLK10 mRNA expression and examine its prognostic value and potential clinical application as a novel molecular tissue biomarker in CRC.Design and methods: The study group consisted of 190 colorectal samples. Total RNA was extracted from pulverised tissues and cDNA was prepared by reverse transcription. KLK10 was amplified by real-time PCR. B2M was used as a reference gene and HT-29 cells as positive control.Results: KLK10 expression was significantly higher in cancer tissues (P < 0.001). Tumours of advanced TNM and Dukes' stage showed high KLK10 expression status (P = 0.036; P = 0.025). Patients with high KLK10 expression had a shorter disease-free and overall survival rates (P = 0.014; P = 0.020).Conclusion: Our results suggest that KLK10 may serve as a new marker of unfavourable prognosis of colorectal cancer. (c) 2013 The Canadian Society of Clinical Chemists. Published by Elsevier Inc. All rights reserved.