The integrative role of orexin/hypocretin neurons in nociceptive perception and analgesic regulation.
The integrative role of orexin/hypocretin neurons in nociceptive perception and analgesic regulation.
复制标题
DOI：
10.1038/srep29480
复制
发表时间：
2016-07-07
影响因子：
4.6
通讯作者：
Yamanaka A
中科院分区：
文献类型：
--
作者：
Inutsuka A;Yamashita A;Chowdhury S;Nakai J;Ohkura M;Taguchi T;Yamanaka A
清醒程度是影响痛觉和疼痛的主要因素之一。应激性镇痛通过对捕食者或竞争者的快速防御反应来支持动物的生存。以往的研究已经证实了食欲素肽对镇痛的药理作用。然而，食欲素神经元不仅含有食欲素，还含有其他共递质，如促啡肽、神经紧张素和谷氨酸。因此，食欲素神经元活动在伤害感觉中的生理重要性尚不清楚。本研究表明，成年期选择性消融食欲素神经元可增强疼痛相关行为，而药物遗传学激活食欲素神经元可诱导镇痛。此外，我们利用纤维光度法记录清醒动物的食欲素神经元，发现在伤害感觉中食欲素神经元的相关激活。这些发现表明食欲素神经元在伤害性知觉和疼痛调节中具有综合作用。
The level of wakefulness is one of the major factors affecting nociception and pain. Stress-induced analgesia supports an animal’s survival via prompt defensive responses against predators or competitors. Previous studies have shown the pharmacological effects of orexin peptides on analgesia. However, orexin neurons contain not only orexin but also other co-transmitters such as dynorphin, neurotensin and glutamate. Thus, the physiological importance of orexin neuronal activity in nociception is unknown. Here we show that adult-stage selective ablation of orexin neurons enhances pain-related behaviors, while pharmacogenetic activation of orexin neurons induces analgesia. Additionally, we found correlative activation of orexin neurons during nociception using fiber photometry recordings of orexin neurons in conscious animals. These findings suggest an integrative role for orexin neurons in nociceptive perception and pain regulation.