The surface display of haemolysin from Vibrio harveyi on yeast cells and their potential applications as live vaccine in marine fish.
The surface display of haemolysin from Vibrio harveyi on yeast cells and their potential applications as live vaccine in marine fish.
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DOI：
10.1016/j.vaccine.2006.05.043
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发表时间：
2006-08
期刊：
影响因子：
5.5
通讯作者：
K. Zhu;Z. Chi;Jing Li;Fengli Zhang;Meiju Li;Hirimuthugoda Nalini Yasoda;Long-Fei Wu
中科院分区：
文献类型：
--
作者：
K. Zhu;Z. Chi;Jing Li;Fengli Zhang;Meiju Li;Hirimuthugoda Nalini Yasoda;Long-Fei Wu
编码哈维氏弧菌SF-1溶血素的HL1基因在酵母细胞中表达，表达的溶血素显示在细胞表面。在含半乳糖的培养液中诱导36h后，1/3的细胞含有展示蛋白，展示细胞对牙鲨红细胞具有溶血活性。双向扩散琼脂分析表明，用展示的具有溶血活性的酵母细胞免疫比目鱼血清，可与纯化的溶血素形成沉淀。ELISA法分析表明，免疫次数对展示的具有溶血活性的酵母细胞免疫大比目鱼产生的溶血素特异性抗体有很大影响。在用哈维氏弧菌SF-1攻击后，用展示的具有溶血活性的酵母细胞免疫比目鱼和大比目鱼，均获得了较早的保护作用和显著的保护作用。这些结果表明，展示的具有溶血活性的酵母细胞可以作为海水鱼的潜在活疫苗。
HL1 gene encoding haemolysin from Vibrio harveyi SF-1 was expressed in yeast cells and the expressed haemolysin was displayed on the cell surface. After induction for 36h in galactose-containing medium, one-third of the cells contained the displayed protein and the displayed cells had haemolytic activity on erythrocytes from flounder. The double diffusion agar analysis showed that the sera from the flounder immunized with the displayed yeast cells having the haemolytic activity could form precipitate with the purified haemolysin. ELISA analysis indicated that immunization times had great influence on increased production of the specific antibody against haemolysin in turbot immunized with the displayed yeast cells having the haemolytic activity. After the challenge with V. harveyi SF-1, it was found that earlier protection in flounder and significant protection in turbot, both of which were immunized with the displayed yeast cells having the haemolytic activity, were achieved. These results suggested that the displayed yeast cells with the haemolytic activity could be used as potential live vaccine in marine fish.