Hesperetin Modifies the Composition of Fecal Microbiota and Increases Cecal Levels of Short-Chain Fatty Acids in Rats
Hesperetin Modifies the Composition of Fecal Microbiota and Increases Cecal Levels of Short-Chain Fatty Acids in Rats
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DOI：
10.1021/acs.jafc.5b02649
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发表时间：
2015-09-16
影响因子：
6.1
通讯作者：
Takahashi, Shunsuke
中科院分区：
文献类型：
--
作者：
Unno, Tomonori;Hisada, Takayoshi;Takahashi, Shunsuke
人们对常食用的多酚类益生元的作用特别感兴趣。本研究旨在探讨柑橘类水果中主要黄酮类化合物橙皮苷（HD）及其苷元橙皮素（HT）对大鼠肠道微生物群结构和活性的影响。大鼠进食指定饮食（对照饮食、0.5% HT饮食或1.0% HD饮食）3周。终端限制性内切片段长度多态性分析显示，HT饲粮显著降低了饲喂第3周粪便中抑制梭菌XIVa的比例，对照组为19.8 +/- 4.3%，HT饲粮为5.3 +/- 1.5% （P < 0.01）。对照饲粮（212 +/- 71 μ mol）和高温饲粮（310 +/- 51 μ mol）对盲肠短链脂肪酸（SCFA）、乙酸、丙酸和丁酸总量有显著影响（P < 0.05），而高温饲粮（245 +/- 51 μ mol）对盲肠短链脂肪酸无显著影响（P < 0.05）。有趣的是，饲粮HT导致粪便中淀粉的排泄量显著增加。HT可能会减少淀粉的消化，而HD则不会，部分未消化的淀粉在大肠内通过微生物发酵产生短链脂肪酸。
There has been particular interest in the prebiotic-like effects of commonly consumed polyphenols. This study aimed to evaluate the effects of hesperidin (HD) and its aglycone hesperetin (HT), major flavonoids in citrus fruits, on the structure and activity of gut microbiota in rats. Rats ingested an assigned diet (a control diet, a 0.5% HT diet, or a 1.0% HD diet) for 3 weeks. Terminal restriction fragment length polymorphism analysis revealed that the proportion of Clostridium subduster XIVa in the feces collected at the third week of feeding was significantly reduced by the HT diet: 19.8 +/- 4.3% for the control diet versus 5.3 +/- 1.5% for the HT diet (P < 0.01). There was a significant difference in the cecal pool of short-chain fatty acids (SCFA), the sum of acetic, propionic, and butyric acids, between the control diet (212 +/- 71 mu mol and the HT diet (310 +/- 51 mu mol) (P < 0.05), whereas the HD diet exhibited no effects (245 +/- 51 mu mol). Interestingly, dietary HT resulted in a significant increase in the excretion of starch in the feces. HT, but not HD, might reduce starch digestion, and parts of undigested starch were utilized to produce SCFA by microbial fermentation in the large intestine.