Incorporation of stromal cell-derived factor-1α in PCL/gelatin electrospun membranes for guided bone regeneration
Incorporation of stromal cell-derived factor-1α in PCL/gelatin electrospun membranes for guided bone regeneration
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DOI：
10.1016/j.biomaterials.2012.10.016
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发表时间：
2013-01-01
期刊：
影响因子：
14
通讯作者：
Jansen, John A.
中科院分区：
文献类型：
--
作者：
Ji, Wei;Yang, Fang;Jansen, John A.
这项工作的目标是评估趋化因子膜功能化对细胞招募和骨形成的影响，以开发用于引导骨再生（GBR）应用的生物活性膜。为此。 GBR 膜采用与 B 型明胶混合的聚（ε-己内酯）（PCL）通过静电纺丝制备，并通过物理吸附用基质细胞衍生因子 1 α（SDF-1 α）进行功能化。首先，在体外评估所获得的膜的 SDF-1 α 释放和对骨髓基质细胞 (BMSC) 的趋化作用。随后，在大鼠颅骨缺损中评估了体内 BMSC 招募和骨再生对 SDF-1 α 负载 PCL/明胶电纺膜的反应。结果表明，PCL/明胶电纺膜提供了扩散控制的 SDF-1 α 释放曲线。此外，负载不同量的SDF-1α（50-400ng）的膜在体外显着诱导BMSCs的趋化性迁移，且没有剂量依赖性效应。植入大鼠颅骨缺损八周后，与裸膜相比，SDF-1 α负载膜的骨形成量增加了6倍，尽管无法确定体内BMSC募集对骨再生的贡献。总之，当前的研究结果表明使用SDF-1 α负载的PCL/明胶电纺膜作为生物活性膜的潜力，这有利于优化GBR策略的临床应用。 (C) 2012 Elsevier Ltd. 保留所有权利。
The goal of this work was to evaluate the effect of membrane functionalization with a chemotactic factor on cell recruitment and bone formation in order to develop a bioactive membrane for guided bone regeneration (GBR) applications. To this end. GBR membranes were prepared by electrospinning using poly(epsilon-caprolactone) (PCL) blended with type B-gelatin, and functionalized with stromal cell derived factor-1 alpha (SDF-1 alpha) via physical adsorption. Firstly, the obtained membranes were evaluated in vitro for SDF-1 alpha release and chemotactic effect on bone marrow stromal cells (BMSCs). Subsequently, in vivo BMSCs recruitment and bone regeneration in response to SDF-1 alpha loaded PCL/gelatin electrospun membranes were assessed in rat cranial defects. The results showed that PCL/gelatin electrospun membranes provided a diffusion-controlled SDF-1 alpha release profile. Furthermore, the membranes loaded with different amounts of SDF-1 alpha (50-400 ng) significantly induced stimulated chemotactic migration of BMSCs in vitro without dose-dependent effects. Eight weeks after implantation in rat cranial defects, SDF-1 alpha loaded membranes yielded a 6-fold increase in the amount of bone formation compared to the bare membranes, albeit that contribution of in vivo BMSCs recruitment to the bone regeneration could not be ascertained. In conclusion, the results of current study indicate the potential for using SDF-1 alpha loaded PCL/gelatin electrospun membrane as a bioactive membrane, which is beneficial for optimizing clinical application of GBR strategies. (C) 2012 Elsevier Ltd. All rights reserved.