Co-expression of two perivascular cell markers isolates mesenchymal stem-like cells from human endometrium
Co-expression of two perivascular cell markers isolates mesenchymal stem-like cells from human endometrium
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DOI：
10.1093/humrep/dem265
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发表时间：
2007-11-01
影响因子：
6.1
通讯作者：
Gargett, C. E.
中科院分区：
文献类型：
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作者：
Schwab, K. E.;Gargett, C. E.
背景：人类子宫内膜具有巨大的再生能力，每月7天内生长约5 mm。我们以前已经确定了一个小群体的集落形成子宫内膜间质细胞，我们假设是间充质干细胞（MSC）。本研究的目的是确定两种血管周围细胞标志物CD 146和血小板衍生生长因子受体β（PDGF-R β）的共表达是否能前瞻性地分离出具有MSC特性的子宫内膜间质细胞，并确定它们在人子宫内膜中的位置。方法：人子宫内膜间质细胞的单细胞悬浮液经荧光激活细胞分选（FACS）分选为CD 146（+）PDGF-R β（+）和CD 146（-）PDGF-R β（-）群体，并分析集落形成能力、体外分化和典型MSC标志物的表达。对全厚度人子宫内膜切片进行CD 146和PDGF-R β共染色。研究结果：与CD 146（-）PDGF-R β（-）细胞相比，FACS基质CD 146（+）PDGF-R β（+）基质细胞（分选群体的1.5%）富集集落形成细胞（7.7 +/- 1.7 vs 0.7 +/-0.2%P < 0.0001），并且还经历分化成脂肪形成、成骨形成、成肌形成和软骨形成谱系。它们表达MSC表型表面标志物，并位于血管附近。结论：这项研究表明，人子宫内膜含有一小群MSC样细胞，可能是其周期性生长的原因，并可能为组织工程应用提供一个现成的MSC来源。
BACKGROUND: Human endometrium has immense regenerative capacity, growing similar to 5 mm in 7 days every month. We have previously identified a small population of colony-forming endometrial stromal cells which we hypothesize are mesenchymal stem cells (MSC). The aim of this study was to determine if the co-expression of two perivascular cell markers, CD146 and platelet-derived growth factor-receptor beta (PDGF-R beta), will prospectively isolate endometrial stromal cells which exhibit MSC properties, and determine their location in human endometrium. METHODS: Single cell suspensions of human endometrial stromal cells were fluorescence activated cell sorting (FACS) sorted into CD146(+)PDGF-R beta(+) and CD146(-)PDGF-R beta(-) populations and analysed for colony-forming ability, in vitro differentiation and expression of typical MSC markers. Full thickness human endometrial sections were co-stained for CD146 and PDGF-R beta. RESULTS: FACS stromal CD146(+)PDGF-R beta(+) stromal cells (1.5% of sorted population) were enriched for colony-forming cells compared with CD146(-)PDGF-R beta(-) cells (7.7 +/- 1.7 versus 0.7 +/- 0.2% P < 0.0001), and also underwent differentiation into adipogenic, osteogenic, myogenic and chondrogenic lineages. They expressed MSC phenotypic surface markers and were located near blood vessels. CONCLUSION: This study shows that human endometrium contains a small population of MSC-like cells that may be responsible for its cyclical growth, and may provide a readily available source of MSC for tissue engineering applications.