Induction of G2/M phase cell cycle arrest by carnosol and carnosic acid is associated with alteration of cyclin A and cyclin B1 levels
Induction of G2/M phase cell cycle arrest by carnosol and carnosic acid is associated with alteration of cyclin A and cyclin B1 levels
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DOI：
10.1016/j.canlet.2005.05.045
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发表时间：
2006-06-08
期刊：
影响因子：
9.7
通讯作者：
Padfield, Philip J.
中科院分区：
文献类型：
--
作者：
Visanji, James M.;Thompson, David G.;Padfield, Philip J.
鼠尾草酚和鼠尾草酸是迷迭香（迷迭香）中存在的两种抗氧化多酚，研究人员研究了它们对 Caco-2 细胞的抗增殖特性。用鼠尾草酚和鼠尾草酸处理 20 小时，以剂量依赖性方式抑制 H-3-胸苷掺入，50% 抑制浓度为 23 μM，并且将 Caco-2 细胞的倍增时间分别从 29.5 小时显着增加到 140 小时和 120 小时。这些效应与细胞周期 G(2)/M 期处理细胞的积累有关。研究发现鼠尾草酚在中期后发挥其主要的细胞周期作用，并导致细胞周期蛋白 B1 蛋白水平增加，而鼠尾草酸在中期前抑制细胞，并导致细胞周期蛋白 A 水平降低。因此，这些结构相关的植物化学物质似乎可能通过影响不同细胞周期蛋白的水平来将细胞阻滞在细胞周期的不同阶段。 (c) 2005 Elsevier Ireland Ltd. 保留所有权利。
Carnosol and carnosic acid, two antioxidant polyphenols present in Rosmarinus officinalis (rosemary), were investigated for their antiproliferative properties toward Caco-2 cells. Twenty hours of treatment with both carnosol and carnosic acid inhibited H-3-thymidine incorporation in a dose-dependent manner, with a 50% inhibitory concentration of 23 mu M and significantly increased the doubling time of Caco-2 cells from 29.5 to 140 and 120 h, respectively. These effects were associated with accumulation of treated cells in the G(2)/M phase of the cell cycle. Carnosol was found to exert its major cell cycle effect after prometaphase, and caused an increase in cyclin B1 protein levels whereas carnosic acid arrested cells prior to prometaphase, and caused a reduction in cyclin A levels. These structurally related phytochemicals, therefore, appear to arrest cells at different phases of the cell cycle possibly through influencing the levels of different cyclin proteins. (c) 2005 Elsevier Ireland Ltd. All rights reserved.