Arg-gingipain a DNA vaccine prevents alveolar bone loss in mice
Arg-gingipain a DNA vaccine prevents alveolar bone loss in mice
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DOI：
10.1177/154405910708600511
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发表时间：
2007-05-01
影响因子：
7.6
通讯作者：
Okuda, K.
中科院分区：
文献类型：
--
作者：
Miyachi, K.;Ishihara, K.;Okuda, K.
牙龈卟啉单胞菌的一个主要致病因子是精氨酸牙龈卟啉菌蛋白酶（Arg-gingipain，Rgp），一种精氨酸特异性半胱氨酸蛋白酶。为了阐明rgpA DNA疫苗的效果，我们用基因枪经腹部或经鼻腔每周免疫BALB/c小鼠，持续6周。免疫后，用牙龈卟啉单胞菌经口攻击小鼠。免疫接种在两组中都引起了针对牙龈卟啉单胞菌的IgG应答。鼻免疫也诱导针对牙龈卟啉单胞菌的sIgA，尽管基因枪免疫没有。在初次感染后42天，两组均观察到牙槽骨丢失减少。鼻内免疫组的这种效果比基因枪组更明显。本研究结果提示rgpA DNA疫苗鼻腔免疫是预防牙龈卟啉单胞菌感染引起牙槽骨丢失的有效方法。
One major pathogenic factor of Porphyromonas gingivalis is Arg-gingipain ( Rgp), an arginine-specific cysteine proteinase. To clarify the effect of rgpA DNA vaccine, we immunized BALB/c mice via the abdomen with a Gene Gun or via the nasal cavity weekly for 6 weeks. After immunization, the mice were challenged orally with P. gingivalis. Immunization elicited IgG responses against P. gingivalis in both groups. Nasal immunization also induced sIgA against P. gingivalis, although Gene Gun immunization did not. Reduction of alveolar bone loss was observed in both groups at 42 days following initial infection. This effect was more pronounced in the intranasal immunization group than in the Gene Gun group. The results of this study suggest that immunization with rgpA DNA vaccine via the nasal cavity is an effective method for preventing alveolar bone loss incurred by infection with P. gingivalis.