Isolation, identification and synthesis of four novel antioxidant peptides from rice residue protein hydrolyzed by multiple proteases.
Isolation, identification and synthesis of four novel antioxidant peptides from rice residue protein hydrolyzed by multiple proteases.
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DOI：
10.1016/j.foodchem.2015.01.137
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发表时间：
2015-07
期刊：
影响因子：
8.8
通讯作者：
Q. Yan;Linhua Huang;Qian Sun;Zhengqiang Jiang;Xia Wu
中科院分区：
文献类型：
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作者：
Q. Yan;Linhua Huang;Qian Sun;Zhengqiang Jiang;Xia Wu
以米渣蛋白为原料，优化复合蛋白酶水解条件，制备新型抗氧化肽。采用膜超滤、体积排阻色谱和反相FPLC从RRP水解物中纯化出IC 50为0.25 mg/ml的抗氧化肽级分（RRPB 3）。RRPB 3由4个肽段组成（RRPB 3 I-IV），氨基酸序列分别为RPNYTDA（835.9 Da）、TSQLLSDQ（891.0 Da）、TRTGDPFF（940.0 Da）和NFHPQ（641.7 Da）。采用化学方法合成了4种抗氧化肽，并分别采用DPPH自由基清除法、ABTS自由基清除法和FRAP-Fe ~（3+）还原法测定了它们的抗氧化活性。RRPB 3 I和III两者与它们各自单独使用相比显示出协同抗氧化活性。所有四种合成肽对模拟胃肠道蛋白酶显示出优异的稳定性。因此，从RRP中分离的肽可以用作食品和药物工业中潜在的抗氧化剂。
Multiple proteases were optimized to hydrolyze the rice residue protein (RRP) to produce novel antioxidant peptides. An antioxidant peptide fraction (RRPB3) with IC50of 0.25 mg/ml was purified from the RRP hydrolysate using membrane ultrafiltration followed by size exclusion chromatography and reversed-phase FPLC. RRPB3 was found to include four peptides (RRPB3 I–IV) and their amino acid sequences were RPNYTDA (835.9 Da), TSQLLSDQ (891.0 Da), TRTGDPFF (940.0 Da) and NFHPQ (641.7 Da), respectively. Furthermore, four peptides were chemically synthesized and their antioxidant activities were assessed by DPPH radical scavenging, ABTS radical scavenging assay and FRAP-Fe3+reducing assay, respectively. Both RRPB3 I and III showed synergistic antioxidant activity compared to each of them used alone. All four synthetic peptides showed excellent stability against simulated gastrointestinal proteases. Therefore, the peptides isolated from RRP may be used as potential antioxidants in the food and drug industries.