Community-associated and healthcare-associated methicillin-resistant Staphylococcus aureus virulence toward Caenorhabditis elegans compared
Community-associated and healthcare-associated methicillin-resistant Staphylococcus aureus virulence toward Caenorhabditis elegans compared
复制标题
DOI：
10.4161/viru.22120
复制
发表时间：
2012-11-01
期刊：
影响因子：
5.2
通讯作者：
Sifri, Costi D.
中科院分区：
文献类型：
--
作者：
Day, Shandra R.;Moore, Christopher M.;Sifri, Costi D.
社区相关（CA）耐甲氧西林金黄色葡萄球菌（MRSA）菌株已成为主要的人类病原体。CA-MRSA毒力似乎与医疗保健相关（HA）MRSA不同，有几种因子[α-溶血素（Hla）、Panton-Valentine杀白细胞素（PVL）、α-型酚可溶性调节蛋白（PSM α）和SCCmec IV]被认为可增强毒力或适应性。使用秀丽隐杆线虫感染模型，我们比较了CA-MRSA和HA-MRSA的临床和实验室分离株的毒力，并探讨了CA-MRSA相关毒力因子对线虫杀灭的贡献。所有CA-MRSA菌株对线虫都具有高致病性，而HA-MRSA菌株表现出可变的线虫杀灭作用。hla或PVL、PSM α、PSM β、PSM δ或SCCmec IV位点的等基因突变体杀死线虫与亲本菌株没有差异。这些结果表明CA-MRSA是高毒力的，表明HA-MRSA的一些菌株对线虫具有相同的毒力，并表明CA-MRSA在C.秀丽隐杆线虫与单一的毒力因子无关。
Community-associated (CA) methicillin-resistant Staphylococcus aureus (MRSA) strains have emerged as major human pathogens. CA-MRSA virulence appears to be distinct from healthcare-associated (HA) MRSA with several factors [alpha-hemolysin (Hla), Panton-Valentine leukocidin (PVL), alpha-type phenol soluble modulins (PSM alpha) and SCCmec IV] postulated to enhance virulence or fitness. Using the Caenorhabditis elegans infection model, we compared the virulence of clinical and laboratory isolates of CA-MRSA and HA-MRSA and explored the contribution of CA-MRSA associated virulence factors to nematode killing. All CA-MRSA strains were highly pathogenic to nematodes, while HA-MRSA strains demonstrated variable nematode killing. Nematode killing by isogenic mutants of hla or the loci for PVL, PSM alpha, PSM beta, PSM delta or SCCmec IV was not different than the parental strains. These results demonstrate that CA-MRSA is highly virulent, shows some strains of HA-MRSA are equally virulent toward nematodes and suggests CA-MRSA virulence in C. elegans is not linked to a single virulence factor.