Merging Microfluidics with Micro-titre Technology for More Efficient Drug Discovery.
Merging Microfluidics with Micro-titre Technology for More Efficient Drug Discovery.
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将微流控技术与微滴定技术相结合，实现更高效的药物发现。
DOI：
10.1016/j.jala.2008.05.002
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发表时间：
2008
期刊：
影响因子：
--
通讯作者：
Spence,DanaM
中科院分区：
文献类型：
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作者：
Tolan,NicoleV;Genes,LuizaI;Spence,DanaM
在基于流的系统中在不到 20 分钟内检测单个红细胞 (RBC) 样品中的多种成分将能够改进药物功效和细胞对所施用药物的反应的体外测定。此处，通过将多种细胞类型整合到单个装置上，确定了涉及伊洛前列素（据报道可改善血流的药物）的改进体外测量的示例。该方法允许流体流向包含在 18 孔微流体阵列内的各行孔，该阵列用作 96 孔微量滴定板装置的前体。通过结合血液成分的流动来更好地模拟体内循环的能力，再加上微量滴定板的同步检测和实验室自动化，表明这里介绍的微流体阵列将允许改进机械药物研究。使用荧光显微镜，还可以使用微流体阵列测定红细胞内存在的多种代谢物的浓度。本文介绍了使用该设备进行个性化医疗的当前进展。
Detecting multiple components from a single red blood cell (RBC) sample within a flow-based system in less than 20 min will enable improved in vitro determinations of drug efficacy and cellular response to administered drugs. Here, an example of an improved in vitro measurement involving iloprost, a pharmaceutical reported to improve blood flow, has been determined by incorporating multiple cell types onto a single device. The method allows fluid flow to address individual rows of wells contained within an 18-well microfluidic array that serves as a precursor to a 96-well microtitre plate device. The ability to better mimic the in vivo circulation by incorporating the flow of blood components, coupled with simultaneous detection and laboratory automation in place for microtitre plates, suggests that the microfluidic array presented here will allow for improved mechanistic drug research studies. Using fluorescence microscopy, concentrations of multiple metabolites present within the RBC can also be determined using the microfluidic array. The current progress toward using this device for personalized medicine is presented here.