Spidey: A tool for mRNA-to-genomic alignments
Spidey: A tool for mRNA-to-genomic alignments
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DOI：
10.1101/gr.195301
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发表时间：
2001-11-01
期刊：
影响因子：
7
通讯作者：
Ostell, JM
中科院分区：
文献类型：
--
作者：
Wheelan, SJ;Church, DM;Ostell, JM
我们已经开发了一个计算机程序，将剪接序列与基因组序列进行比对，使用局部比对算法和启发式算法将全局剪接比对放在一起。即使面对来自不同剪接、多态性、测序错误或进化分歧的干扰，蜘蛛也能快速产生可靠的比对。我们展示了如何使用蜘蛛将参考序列与已知的基因组序列比对，然后将其与人类基因组草图比对，将mrna与基因簇比对，并将小鼠mrna与人类基因组序列比对。我们将蜘蛛侠与其他两个拼接对齐程序进行了比较；蜘蛛侠通常在相当合理的时间内表现得相当好。
We have developed a computer program that aligns spliced sequences to genomic Sequences, using local alignment algorithms and heuristics to Put together a global spliced alignment. Spidey can produce reliable alignments quickly, even when confronted with noise from alternative splicing, polymorphisms, Sequencing errors, or evolutionary divergence. We show how Spidey was used to align reference sequences to known genomic sequences and then to the draft human genome, to align mRNAs to gene clusters, and to align mouse mRNAs to human genomic sequence. We compared Spidey to two other spliced alignment programs; Spidey generally performed quite well in a very reasonable amount of time.