Transitions in cardiac isomyosin expression during differentiation of the embryonic chick heart.
Transitions in cardiac isomyosin expression during differentiation of the embryonic chick heart.
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胚胎鸡心脏分化过程中心脏异肌球蛋白表达的转变。
DOI：
10.1161/01.res.61.2.287
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发表时间：
1987
影响因子：
20.1
通讯作者：
Manasek,FJ
中科院分区：
文献类型：
--
作者：
Sweeney,LJ;Zak,R;Manasek,FJ
不同亚型的收缩蛋白肌球蛋白的表达在决定成人心肌和骨骼肌的收缩特性中起着重要作用。几乎没有证据表明心脏胚胎发生期间肌球蛋白表型发生了假定的变化，或者这种变化是否可以在调节发育中心脏的收缩特征方面发挥作用。我们研究了异肌球蛋白的表达在鸡心脏发生的间接免疫荧光显微镜与成人心室和心房肌球蛋白重链的单克隆抗体。免疫荧光定位、ELISA和蛋白质印迹免疫分析的基础上，在成人中的抗体特异性。结果表明，早期胚胎鸡心脏具有不同的肌球蛋白表型比晚期胚胎或成人心脏。胚胎心室和心房心肌最初表达的肌球蛋白重链被识别的抗体特异性（在成人）心室肌球蛋白重链。心室在整个生命过程中与心室抗体保持反应性，但心房心肌的反应性仅限于胚胎发育的最初6天。另一方面，在整个发育过程中，胚胎心脏与心房肌球蛋白的多种特异性抗体（在成人中）的反应仅限于心房心肌。因此，肌球蛋白异构体的分布变得类似于成人心肌的时间，胚胎心脏达到4室配置在6天的卵。
The expression of different isoforms of the contractile protein myosin plays a major role in determining contractile characteristics in both cardiac and skeletal muscle in the adult. There is little evidence pertaining to putative changes in myosin phenotype during cardiac embryogenesis or if such changes could play a role in modulating the contractile characteristics of the developing heart. We examined isomyosin expression during cardiogenesis in the chick by indirect immunofluorescence microscopy with monoclonal antibodies to adult ventricular and atrial myosin heavy chains. Antibody specificity was characterized in the adult on the basis of immunofluorescence localization, ELISA, and protein blot immunoassay. Results show that the early embryonic chick heart has a different myosin phenotype than the later embryonic or adult heart. Both the embryonic ventricular and atrial myocardia initially expressed a myosin heavy chain that was recognized by antibody specific (in the adult) for ventricular myosin heavy chain. The ventricles remained reactive throughout life with the ventricular antibody, but reactivity of the atrial myocardium was confined to the initial 6 days of embryonic development. On the other hand, reactivity of the embryonic heart with multiple antibodies specific (in the adult) for atrial myosin was confined to the atrial myocardium throughout development. Thus, the distribution of myosin isoforms became similar to that of the adult myocardium by the time the embryonic heart achieved a 4-chambered configuration at 6 days in ovo.