Induction of tissue- and stressor-specific kinomic responses in chickens exposed to hot and cold stresses
Induction of tissue- and stressor-specific kinomic responses in chickens exposed to hot and cold stresses
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DOI：
10.3382/ps/pev046
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发表时间：
2015-06-01
期刊：
影响因子：
4.4
通讯作者：
Shand, Phyllis
中科院分区：
文献类型：
--
作者：
Napper, Scott;Dadgar, Samira;Shand, Phyllis
在全球细胞激酶(Kinome)活性水平上定义细胞反应是破译复杂生物学和识别生物标记物的有效方法。在这里，我们报道了鸡特异性多肽阵列的开发，并将其应用于描述家禽胸部(胸大肌)和大腿(髂胫肌)肌肉对温度应激的动态组反应，以模拟鸟类在商业运输过程中所经历的条件。胸部和大腿肌肉表现出独特的基因组轮廓，突出了这些组织的独特性质。在这些不同的背景下，观察到了特定于组织和温度的动态组反应。在乳房，冷和热应激都激活了钙依赖的代谢适应。在乳房内，也有ErbB信号的激活，以及AMPK磷酸化的动态模式，AMPK是代谢的关键调节酶，但仅限于冷应激。在大腿，冷应激诱导的反应暗示着组织损伤的发生，包括激活先天免疫信号通路和组织修复通路(转化生长因子-β)。相比之下，大腿热应激通过脂肪细胞因子和哺乳动物雷帕霉素靶点(MTOR)信号通路激活与蛋白质和脂肪代谢相关的通路。通过传统的pH、糖酵解潜力和肉类品质指标来确定这些组织对这些压力的反应，提供了组织和应激源特异性反应的类似结论，验证了基因组结果。总而言之，这项研究的结果突出了乳房和大腿组织对热和冷应激的独特细胞反应，并可能为深入了解这些组织对热应激的独特易感性以及功能后果提供帮助。
Defining cellular responses at the level of global cellular kinase (kinome) activity is a powerful approach to deciphering complex biology and identifying biomarkers. Here we report on the development of a chicken-specific peptide array and its application to characterizing kinome responses within the breast (pectoralis major) and thigh (iliotibialis) muscles of poultry subject to temperature stress to mimic conditions experienced by birds during commercial transport. Breast and thigh muscles exhibited unique kinome profiles, highlighting the distinct nature of these tissues. Against these distinct backgrounds, tissue- and temperature-specific kinome responses were observed. In breast, both cold and hot stresses activated calcium-dependent metabolic adaptations. Also within breast, but specific to cold stress, was the activation of ErbB signaling as well as dynamic patterns of phosphorylation of AMPK, a key regulatory enzyme of metabolism. In thigh, cold stress induced responses suggestive of the occurrence of tissue damage, including activation of innate immune signaling pathways and tissue repair pathways (TGF-beta). In contrast, heat stress in thigh activated pathways associated with protein and fat metabolism through adipocytokine and mammalian target of rapamycin (mTOR) signaling. Defining the responses of these tissues to these stresses through conventional markers of pH, glycolytic potential, and meat quality offered a similar conclusion of the tissue- and stressor-specific responses, validating the kinome results. Collectively, the results of this study highlight the unique cellular responses of breast and thigh tissues to heat and cold stresses and may offer insight into the unique susceptibilities, as well as functional consequences, of these tissues to thermal stress.