Isolation, characterization of Rhodococcus sp P14 capable of degrading high-molecular-weight polycyclic aromatic hydrocarbons and aliphatic hydrocarbons

Isolation, characterization of Rhodococcus sp P14 capable of degrading high-molecular-weight polycyclic aromatic hydrocarbons and aliphatic hydrocarbons
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DOI:
10.1016/j.marpolbul.2011.07.013
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发表时间:
2011-10-01
影响因子:
5.8
通讯作者:
Hu, Zhong
Hu, Zhong
中科院分区:
环境科学与生态学3区
文献类型:
--
作者:
Song, Xiaohui;Xu, Yan;Hu, Zhong

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红球菌属P14是从原油污染的沉积物中分离出来的。该菌株能够利用菲(Phe)、芘(PYR)和苯并[a]芘(BaP)等三到五环多环芳烃(PAHs)作为唯一的碳源和能源。菌株P14经50 mg/L的多环芳烃培养后,30d内对43%Phe、34%PYR和30%BaP的降解率分别为43%、34%和30%。菌株P14还能降解碳链长度为C17~C21的正构烷烃矿物油。与葡萄糖培养的细胞相比。PAHs培养的细胞中较短链长的脂肪酸含量降低(
Rhodococcus sp. P14 was isolated from crude oil-contaminated sediments. This strain was capable of utilizing three to five rings polycyclic aromatic hydrocarbons (PAHs) including phenanthrene (Phe), pyrene (Pyr), and benzo[a]pyrene (BaP) as a sole carbon and energy source. After cultivated with 50 mg/L of each PAH, strain P14 removed 43% Phe, 34% Pyr and 30% BaP in 30 d. Four different hydroxyphenanthrene products derived from Phe by strain P14 (1,2,3,4-hydroxyphenanthrene) were detected using SPME-GC-MS. Strain P14 also was capable of degrading mineral oil with n-alkanes of C17 to C21 carbon chain length. Compared with glucose-grown cells. PAHs-grown cells had decreased contents of shorter-chain length fatty acids (