Repository : Kernel A Single-Batch Fermentation System to Simulate Human Colonic Microbiota for High-Throughput Evaluation of Prebiotics
Repository : Kernel A Single-Batch Fermentation System to Simulate Human Colonic Microbiota for High-Throughput Evaluation of Prebiotics
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存储库：模拟人类结肠微生物群的单批次发酵系统，用于益生元的高通量评估
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G. Kelly
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作者：
G. Kelly
我们设计了一种单批发酵系统，用于从粪便样本中模拟人类结肠微生物群，使微生物的复杂混合物在24小时内达到高达10¹¹个细胞/毫升的密度。对该系统中生长的细菌进行的16S rRNA基因序列分析显示，主要门类的代表，包括拟杆菌门、厚壁菌门和放线菌门，以及整体的物种多样性，与原始粪便中的情况一致。在发酵的早期阶段（长达9小时），可以检测到乙酸盐、乳酸盐和琥珀酸盐的微量混合物；在后期阶段（24小时后），大量的乙酸盐与一些丙酸盐和丁酸盐一起积累。这些模式与在原始粪便中观察到的模式相似。因此，该系统可以作为一个简单的模型来模拟人类结肠微生物群的多样性以及代谢。向该系统中添加几种益生元低聚糖（包括果聚糖、半乳聚糖、异麦芽聚糖和木聚糖低聚糖；乳果糖；以及乳糖蔗糖）导致双歧杆菌属的种群增加，同时乙酸产量显著增加。结果表明，该发酵系统可能有助于在人体试验之前对益生元的效果进行体外临床前评估。发酵的启动。香农 - 维纳多样性指数描述了一个群落的多样性，即物种丰富度和均匀度。
We devised a single-batch fermentation system to simulate human colonic microbiota from fecal samples, enabling the complex mixture of microorganisms to achieve densities of up to 10 11 cells/mL in 24 h. 16S rRNA gene sequence analysis of bacteria grown in the system revealed that representatives of the major phyla, including Bacteroidetes, Firmicutes, and Actinobacteria, as well as overall species diversity, were consistent with those of the original feces. On the earlier stages of fermentation (up to 9 h), trace mixtures of acetate, lactate, and succinate were detectable; on the later stages (after 24 h), larger amounts of acetate accumulated along with some of propionate and butyrate. These patterns were similar to those observed in the original feces. Thus, this system could serve as a simple model to simulate the diversity as well as the metabolism of human colonic microbiota. Supplementa-tion of the system with several prebiotic oligosaccharides (including fructo-, galacto-, iso-malto-, and xylo-oligosaccharides; lactulose; and lactosucrose) resulted in an increased population in genus Bifidobacterium , concomitant with significant increases in acetate production. The results suggested that this fermentation system may be useful for in vitro , pre-clinical evaluation of the effects of prebiotics prior to testing in humans. the initiation of fermentation. The Shannon-Wiener diversity index characterizes the diversity in a community, i.e., species abundance and evenness.