Effect of α-asarone on ethanol-induced learning and memory impairment in mice and its underlying mechanism
Effect of α-asarone on ethanol-induced learning and memory impairment in mice and its underlying mechanism
复制标题
α-细辛脑对乙醇所致小鼠学习记忆障碍的影响及其机制
DOI：
10.1016/j.lfs.2019.116898
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发表时间：
2019-12-01
期刊：
影响因子：
6.1
通讯作者：
He，Wenbin
中科院分区：
文献类型：
--
作者：
Li，Qinqing;Xu，Feifei;He，Wenbin
目的学习记忆障碍是各类痴呆早期的常见症状。通过正确的干预，可能会降低痴呆症的发病率。α-细辛醚是从石菖蒲中分离得到的主要活性物质，已被证实具有改善记忆功能障碍的作用，但其具体作用机制目前尚不清楚。本研究旨在探讨α-细辛醚对乙醇所致小鼠学习记忆障碍的影响及其机制。α-细辛脑（7.5、15或30 mg/kg，i. p.）在给予乙醇之前10分钟递送。采用新物体再认实验评价α-细辛醚的行为学效应。结果：α-细辛醚预处理可明显改善大鼠行为学表现，调节海马Glu和GABA的失衡及相关蛋白的异常表达。一个可能的潜在机制是调节钙信号级联反应，以纠正相关蛋白质的功能，从而维持谷氨酸水平。重要性我们的研究结果表明，α-细辛脑引起的学习和记忆改善可能提供了一种可能的新候选药物用于预防痴呆早期阶段的学习和记忆障碍。
AimsLearning and memory impairment is a common symptom in the early stages of various types of dementia. It is likely to reduce the incidence of dementia with correct intervention. α-Asarone is the main bioactive substance isolated from Acorus tatarinowii Schott and has been proven to improve memory dysfunction; however, at present, the specific underlying mechanism is poorly understood. The aim of the present study was to investigate the effect of α-asarone on ethanol-impaired cognitive ability and explore the underlying mechanism in mice.Main methodsA mouse model of impaired learning and memory was created by ethanol (2.0 g/kg, i.g.). α-Asarone (7.5, 15 or 30 mg/kg, i.p.) was delivered 10 min prior to ethanol administration. The behavioral effect of α-asarone was evaluated using the novel object recognition test. Glutamate (Glu) and γ-aminobutyric acid (GABA) levels in the hippocampus were determined by ELISA, and the protein expression levels of hippocampal GluR2, NMDAR2B, SYNΙ, GLT-1 and CaMKⅡ were detected by western blotting.Key findingsPretreatment with α-asarone significantly improved the behavioral performance, regulated the imbalance of Glu and GABA in the hippocampus and the abnormal expression of related proteins. A possible underlying mechanism is regulation of the calcium signaling cascade to correct functioning of related proteins, and thus, maintain the level of Glu.SignificanceOur results show that the improvement in learning and memory elicited by α-asarone may providing a possible novel candidate for the prevention of learning and memory impairment in the early stages of dementia.