Ewing Sarcoma Cells Secrete EWS/Fli-1 Fusion mRNA via Microvesicles
Ewing Sarcoma Cells Secrete EWS/Fli-1 Fusion mRNA via Microvesicles
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DOI：
10.1371/journal.pone.0077416
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发表时间：
2013-10-04
期刊：
影响因子：
3.7
通讯作者：
Ohno, Takatoshi
中科院分区：
文献类型：
--
作者：
Tsugita, Masanori;Yamada, Nami;Ohno, Takatoshi
肿瘤定义为尤文肉瘤（ES）构成了一组高度恶性肿瘤，最常见的影响儿童和年轻人在前20年的生活。EWS/Fli-1融合基因是t（11;22）（q24;12）易位的产物，在95%的ES患者中检测到。最近，证实了细胞将囊泡、细胞器样结构（微泡，MV）的异质混合物发射到其周围环境（包括血液和体液）中，并且这些MV含有一组选定的肿瘤相关蛋白和高水平的mRNA和miRNA。在本研究中，我们检测了尤文肉瘤特异性EWS/Fli-1 mRNA在来自携带t（11;22）（q24;12）的ES细胞系培养液的MV中的表达。此外，我们在接种TC 135或A673细胞异种移植物的小鼠的约40%的血液样品中检测到这种融合基因。这些结果表明，MVs中EWS/Fli-1 mRNA可能是一种新的非侵入性诊断尤文肉瘤的特定情况下的标志物。
Tumours defined as Ewing sarcoma (ES) constitute a group of highly malignant neoplasms that most often affect children and young adults in the first 2 decades of life. The EWS/Fli-1 fusion gene, a product of the translocation t(11;22) (q24;12), is detected in 95% of ES patients. Recently, it was validated that cells emit a heterogeneous mixture of vesicular, organelle-like structures (microvesicles, MVs) into their surroundings including blood and body fluids, and that these MVs contain a selected set of tumor-related proteins and high levels of mRNAs and miRNAs. In this present study, we detected the Ewing sarcoma-specific EWS/Fli-1 mRNA in MVs from the culture medium of ES cell lines carrying t(11;22) (q24;12). Also, we detected this fusion gene in approximately 40% of the blood samples from mice inoculated with xenografts of TC135 or A673 cells. These findings indicate the EWS/Fli-1 mRNA in MVs might be a new non-invasive diagnostic marker for specific cases of Ewing sarcoma.